Methods in Molecular Biology

The Nuclear Envelope

Methods and Protocols

Editors: Shackleton, Sue, Collas, Philippe, Schirmer, Eric C. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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Hardcover $199.99
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About this book

This volume provides a wide range of protocols used in studying the nuclear envelope, with special attention to the experimental adjustments that may be required to successfully investigate this complex organelle in cells from various organisms. The Nuclear Envelope: Methods and Protocols is divided into five sections: Part I – Nuclear Envelope Isolation; Part II – Nuclear Envelope Protein Interactions, Localization, and Dynamics; Part III – Nuclear Envelope Interactions with the Cytoskeleton; Part IV – Nuclear Envelope-Chromatin Interactions; and Part V – Nucleo-Cytoplasmic Transport. Many of the modifications discussed in this book have only been circulated within laboratories that have conducted research in this field for many years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, The Nuclear Envelope: Methods and Protocols is a timely resource for researchers who have joined this dynamic and rapidly growing field. 

Table of contents (31 chapters)

Table of contents (31 chapters)
  • Isolation, Proteomic Analysis, and Microscopy Confirmation of the Liver Nuclear Envelope Proteome

    Pages 3-44

    Korfali, Nadia (et al.)

  • Exploring the Protein Composition of the Plant Nuclear Envelope

    Pages 45-65

    Zhou, Xiao (et al.)

  • High-Efficiency Isolation of Nuclear Envelope Protein Complexes from Trypanosomes

    Pages 67-80

    Obado, Samson O. (et al.)

  • Superresolution Microscopy of the Nuclear Envelope and Associated Proteins

    Pages 83-97

    Xie, Wei (et al.)

  • Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS)

    Pages 99-111

    Takeshi, Shimi (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-3530-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-3528-4
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8068-0
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
The Nuclear Envelope
Book Subtitle
Methods and Protocols
Editors
  • Sue Shackleton
  • Philippe Collas
  • Eric C. Schirmer
Series Title
Methods in Molecular Biology
Series Volume
1411
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3530-7
DOI
10.1007/978-1-4939-3530-7
Hardcover ISBN
978-1-4939-3528-4
Softcover ISBN
978-1-4939-8068-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 523
Number of Illustrations
26 b/w illustrations, 77 illustrations in colour
Topics