Methods in Molecular Biology

Quantitative Proteomics by Mass Spectrometry

Editors: Sechi, Salvatore (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-3524-6
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About this book

This volume describes prominent methodologies developed by laboratories that have been leading the field of quantitative proteomics by mass spectrometry. The procedures for performing the experiments are described in an easy-to-understand manner with many technical details that usually are not reported in typical research articles. This second edition of Quantitative Proteomics by Mass Spectrometry provides a broad perspective of the methodologies used for quantifying proteins and post-translational modifications in different types of biomedical specimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Quantitative Proteomics by Mass Spectrometry, Second Edition is a valuable resource to help researchers understand and learn about the latest tools used in the study of quantitative proteomics by mass spectrometry.


 

Table of contents (18 chapters)

Table of contents (18 chapters)
  • Increased Depth and Breadth of Plasma Protein Quantitation via Two-Dimensional Liquid Chromatography/Multiple Reaction Monitoring-Mass Spectrometry with Labeled Peptide Standards

    Pages 1-21

    Percy, Andrew J., Ph.D. (et al.)

  • Quantitative Analysis of the Sirt5-Regulated Lysine Succinylation Proteome in Mammalian Cells

    Pages 23-37

    Chen, Yue

  • Determining the Composition and Stability of Protein Complexes Using an Integrated Label-Free and Stable Isotope Labeling Strategy

    Pages 39-63

    Greco, Todd M. (et al.)

  • Label-Free Quantitation for Clinical Proteomics

    Pages 65-76

    Moulder, Robert, Ph.D. (et al.)

  • Proteogenomic Methods to Improve Genome Annotation

    Pages 77-89

    Datta, Keshava K. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-3524-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $139.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Quantitative Proteomics by Mass Spectrometry
Editors
  • Salvatore Sechi
Series Title
Methods in Molecular Biology
Series Volume
1410
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3524-6
DOI
10.1007/978-1-4939-3524-6
Hardcover ISBN
978-1-4939-3522-2
Softcover ISBN
978-1-4939-8066-6
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIII, 306
Number of Illustrations
37 b/w illustrations, 450 illustrations in colour
Topics