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  • © 2016

Quantitative Proteomics by Mass Spectrometry

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1410)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
  2. Label-Free Quantitation for Clinical Proteomics

    • Robert Moulder, Young Ah Goo, David R. Goodlett
    Pages 65-76
  3. Proteogenomic Methods to Improve Genome Annotation

    • Keshava K. Datta, Anil K. Madugundu, Harsha Gowda
    Pages 77-89
  4. Mass Spectrometry-Based Quantitative O-GlcNAcomic Analysis

    • Junfeng Ma, Gerald W. Hart
    Pages 91-103
  5. A Method for Label-Free, Differential Top-Down Proteomics

    • Ioanna Ntai, Timothy K. Toby, Richard D. LeDuc, Neil L. Kelleher
    Pages 121-133
  6. Selecting Optimal Peptides for Targeted Proteomic Experiments in Human Plasma Using In Vitro Synthesized Proteins as Analytical Standards

    • James G. Bollinger, Andrew B. Stergachis, Richard S. Johnson, Jarrett D. Egertson, Michael J. MacCoss
    Pages 207-221
  7. Using the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted Proteomics Assays

    • Jeffrey R. Whiteaker, Goran N. Halusa, Andrew N. Hoofnagle, Vagisha Sharma, Brendan MacLean, Ping Yan et al.
    Pages 223-236
  8. Multiple and Selective Reaction Monitoring Using Triple Quadrupole Mass Spectrometer: Preclinical Large Cohort Analysis

    • Qin Fu, Zhaohui Chen, Shenyan Zhang, Sarah J. Parker, Zongming Fu, Adrienne Tin et al.
    Pages 249-264
  9. Methods for SWATHâ„¢: Data Independent Acquisition on TripleTOF Mass Spectrometers

    • Ronald J. Holewinski, Sarah J. Parker, Andrea D. Matlock, Vidya Venkatraman, Jennifer E. Van Eyk
    Pages 265-279
  10. Measurement of Phosphorylated Peptides with Absolute Quantification

    • Raven J. Reddy, Timothy G. Curran, Yi Zhang, Forest M. White
    Pages 281-292
  11. Back Matter

    Pages 305-306

About this book

This volume describes prominent methodologies developed by laboratories that have been leading the field of quantitative proteomics by mass spectrometry. The procedures for performing the experiments are described in an easy-to-understand manner with many technical details that usually are not reported in typical research articles. This second edition of Quantitative Proteomics by Mass Spectrometry provides a broad perspective of the methodologies used for quantifying proteins and post-translational modifications in different types of biomedical specimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Quantitative Proteomics by Mass Spectrometry, Second Edition is a valuable resource to help researchers understand and learn about the latest tools used in the study of quantitative proteomics by mass spectrometry.


 

Editors and Affiliations

  • NIDDK, National Institutes of Health, BETHESDA, USA

    Salvatore Sechi

Bibliographic Information

  • Book Title: Quantitative Proteomics by Mass Spectrometry

  • Editors: Salvatore Sechi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3524-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3522-2Published: 12 February 2016

  • Softcover ISBN: 978-1-4939-8066-6Published: 31 March 2018

  • eBook ISBN: 978-1-4939-3524-6Published: 11 February 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 306

  • Number of Illustrations: 37 b/w illustrations, 450 illustrations in colour

  • Topics: Protein Science, Mass Spectrometry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access