- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume describes prominent methodologies developed by laboratories that have been leading the field of quantitative proteomics by mass spectrometry. The procedures for performing the experiments are described in an easy-to-understand manner with many technical details that usually are not reported in typical research articles. This second edition of Quantitative Proteomics by Mass Spectrometry provides a broad perspective of the methodologies used for quantifying proteins and post-translational modifications in different types of biomedical specimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Quantitative Proteomics by Mass Spectrometry, Second Edition is a valuable resource to help researchers understand and learn about the latest tools used in the study of quantitative proteomics by mass spectrometry.
- Table of contents (18 chapters)
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Increased Depth and Breadth of Plasma Protein Quantitation via Two-Dimensional Liquid Chromatography/Multiple Reaction Monitoring-Mass Spectrometry with Labeled Peptide Standards
Pages 1-21
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Quantitative Analysis of the Sirt5-Regulated Lysine Succinylation Proteome in Mammalian Cells
Pages 23-37
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Determining the Composition and Stability of Protein Complexes Using an Integrated Label-Free and Stable Isotope Labeling Strategy
Pages 39-63
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Label-Free Quantitation for Clinical Proteomics
Pages 65-76
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Proteogenomic Methods to Improve Genome Annotation
Pages 77-89
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Table of contents (18 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Quantitative Proteomics by Mass Spectrometry
- Editors
-
- Salvatore Sechi
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1410
- Copyright
- 2016
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-3524-6
- DOI
- 10.1007/978-1-4939-3524-6
- Hardcover ISBN
- 978-1-4939-3522-2
- Softcover ISBN
- 978-1-4939-8066-6
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XIII, 306
- Number of Illustrations
- 37 b/w illustrations, 450 illustrations in colour
- Topics
