Save 40% on select Business & Management books + FREE shipping or 50% on Physics eBooks!

Methods in Molecular Biology

Chemotaxis

Methods and Protocols

Editors: Jin, Tian, Hereld, Dale (Eds.)

Free Preview
  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3480-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-3478-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8059-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This second edition expands on the first edition with new chapters describing methods for studying cell movement, molecular components involved in chemotaxis, spatiotemporal dynamics of signaling components, and quantitative modeling, as well as several updated chapters from the first edition. Various methods to investigate directional cell growth and movements are presented in Chapters 1-20. These chapters contains experimental procedures to visualize and measure migration behaviors of different kinds of organisms, including chemotropism in the budding yeast; cell growth and migration of D. discoideum; border cell migration in Drosophila; chemotaxis of mouse and human neutrophils; and HIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols, Second Edition also contains microscopy procedures for studying breast cancer cell migration, tumor cell invasion in vivo, and axon guidance. The book concludes with Chapters 21-27 describing methods that measure spatiotemporal dynamics of signaling components involved in chemotaxis; introduce imaging techniques, such as TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models of experimentally generated chemoattractant gradients. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Chemotaxis: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the diverse methodologies that are propelling chemotaxis research forward. 

Table of contents (27 chapters)

Table of contents (27 chapters)
  • In Situ Assays of Chemotropism During Yeast Mating

    Pages 1-12

    Stone, David E. (et al.)

  • Imaging Polarization in Budding Yeast

    Pages 13-23

    McClure, Allison W. (et al.)

  • Migration of Dictyostelium discoideum to the Chemoattractant Folic Acid

    Pages 25-39

    Aufderheide, Karl J. (et al.)

  • Mitochondrial Stress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells

    Pages 41-61

    Lay, Sui (et al.)

  • Studying Chemoattractant Signal Transduction Dynamics in Dictyostelium by BRET

    Pages 63-77

    Islam, A. F. M. Tariqul (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3480-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-3478-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-8059-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Chemotaxis
Book Subtitle
Methods and Protocols
Editors
  • Tian Jin
  • Dale Hereld
Series Title
Methods in Molecular Biology
Series Volume
1407
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3480-5
DOI
10.1007/978-1-4939-3480-5
Hardcover ISBN
978-1-4939-3478-2
Softcover ISBN
978-1-4939-8059-8
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 425
Number of Illustrations
35 b/w illustrations, 83 illustrations in colour
Topics