Methods in Molecular Biology

Biotechnology of Plant Secondary Metabolism

Methods and Protocols

Editors: Fett-Neto, Arthur Germano (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-3393-8
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About this book

This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal

    Pages 1-18

    Sivanandhan, Ganeshan (et al.)

  • Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction

    Pages 19-26

    Füller, Thanise Nogueira (et al.)

  • A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines

    Pages 27-33

    Lima, Júlio César (et al.)

  • Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves

    Pages 35-42

    Madsen, Svend Roesen (et al.)

  • From Plant Extract to a cDNA Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica

    Pages 43-48

    Costa, Fernanda (et al.)

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-3393-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts from 10 eBooks
Hardcover $159.99
price for USA in USD
Softcover $119.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Biotechnology of Plant Secondary Metabolism
Book Subtitle
Methods and Protocols
Editors
  • Arthur Germano Fett-Neto
Series Title
Methods in Molecular Biology
Series Volume
1405
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3393-8
DOI
10.1007/978-1-4939-3393-8
Hardcover ISBN
978-1-4939-3391-4
Softcover ISBN
978-1-4939-8040-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 180
Number of Illustrations
15 b/w illustrations, 22 illustrations in colour
Topics