Methods in Molecular Biology

Transposons and Retrotransposons

Methods and Protocols

Editors: Garcia-Pérez, Jose L. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume covers the latest protocols designed to identify and characterize TEs in genomes, ancient or recently inserted. Additionally, this book includes a series of protocols designed to understand how host genomes act to regulate the activity of TEs, from elegant genetic mobilization assays to key biochemical methods. Finally, this book also includes chapters that describe how TEs can be used for biotechnological applications. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Transposons and Retrotransposons: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Table of contents (26 chapters)

Table of contents (26 chapters)
  • Study of Transposable Elements and Their Genomic Impact

    Muñoz-Lopez, Martin, Ph.D. (et al.)

    Pages 1-19

  • Bacterial Group II Introns: Identification and Mobility Assay

    Toro, Nicolás, Ph.D. (et al.)

    Pages 21-32

  • In Silico Methods to Identify Exapted Transposable Element Families

    Ramsay, LeeAnn (et al.)

    Pages 33-45

  • Retrotransposon Capture Sequencing (RC-Seq): A Targeted, High-Throughput Approach to Resolve Somatic L1 Retrotransposition in Humans

    Sanchez-Luque, Francisco J. (et al.)

    Pages 47-77

  • Long Interspersed Element Sequencing (L1-Seq): A Method to Identify Somatic LINE-1 Insertions in the Human Genome

    Doucet, Tara T. (et al.)

    Pages 79-93

Buy this book

eBook n/a
  • ISBN 978-1-4939-3372-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-4939-3370-9
  • Free shipping for individuals worldwide
Softcover n/a
  • ISBN 978-1-4939-8033-8
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Transposons and Retrotransposons
Book Subtitle
Methods and Protocols
Editors
  • Jose L. Garcia-Pérez
Series Title
Methods in Molecular Biology
Series Volume
1400
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3372-3
DOI
10.1007/978-1-4939-3372-3
Hardcover ISBN
978-1-4939-3370-9
Softcover ISBN
978-1-4939-8033-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 434
Number of Illustrations
17 b/w illustrations, 56 illustrations in colour
Topics