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Methods in Molecular Biology

Imaging Flow Cytometry

Methods and Protocols

Editors: Barteneva, Natasha S., Vorobjev, Ivan A. (Eds.)

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  • Includes cutting-edge methods and protocols for imaging flow cytometry
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-3302-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for USA in USD
Softcover $119.99
price for USA in USD
About this book

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

Reviews

“The purpose is to present to the scientific community the compendium of recently developed imaging flow cytometry techniques and protocols. The authors are successful in achieving these worthy objectives. Intended for the scientific community, this book will be very useful for students, graduate students, and scientists interested in learning about imaging flow cytometry and its applications. … This book for the first time explores the techniques and protocols involving quantitative imaging flow cytometry in a fascinating manner.” (Omer Iqbal, Doody's Book Reviews, September, 2016)


Table of contents (20 chapters)

Table of contents (20 chapters)
  • Quantitative Functional Morphology by Imaging Flow Cytometry

    Pages 3-11

    Vorobjev, Ivan A. (et al.)

  • Principles of Amnis Imaging Flow Cytometry

    Pages 13-21

    Basiji, David A.

  • Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch

    Pages 23-45

    Lau, Andy K. S. (et al.)

  • Applications of Imaging Flow Cytometry for Microalgae

    Pages 47-67

    Hildebrand, Mark (et al.)

  • The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry

    Pages 71-95

    Filby, Andrew (et al.)

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-3302-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for USA in USD
Softcover $119.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Imaging Flow Cytometry
Book Subtitle
Methods and Protocols
Editors
  • Natasha S. Barteneva
  • Ivan A. Vorobjev
Series Title
Methods in Molecular Biology
Series Volume
1389
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3302-0
DOI
10.1007/978-1-4939-3302-0
Hardcover ISBN
978-1-4939-3300-6
Softcover ISBN
978-1-4939-4901-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 295
Number of Illustrations
3 b/w illustrations, 86 illustrations in colour
Topics