Imaging Flow Cytometry
Methods and Protocols
Editors: Barteneva, Natasha S., Vorobjev, Ivan A. (Eds.)
Free Preview- Includes cutting-edge methods and protocols for imaging flow cytometry
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.
- Reviews
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“The purpose is to present to the scientific community the compendium of recently developed imaging flow cytometry techniques and protocols. The authors are successful in achieving these worthy objectives. Intended for the scientific community, this book will be very useful for students, graduate students, and scientists interested in learning about imaging flow cytometry and its applications. … This book for the first time explores the techniques and protocols involving quantitative imaging flow cytometry in a fascinating manner.” (Omer Iqbal, Doody's Book Reviews, September, 2016)
- Table of contents (20 chapters)
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Quantitative Functional Morphology by Imaging Flow Cytometry
Pages 3-11
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Principles of Amnis Imaging Flow Cytometry
Pages 13-21
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Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch
Pages 23-45
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Applications of Imaging Flow Cytometry for Microalgae
Pages 47-67
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The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry
Pages 71-95
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Table of contents (20 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Imaging Flow Cytometry
- Book Subtitle
- Methods and Protocols
- Editors
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- Natasha S. Barteneva
- Ivan A. Vorobjev
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1389
- Copyright
- 2016
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-3302-0
- DOI
- 10.1007/978-1-4939-3302-0
- Hardcover ISBN
- 978-1-4939-3300-6
- Softcover ISBN
- 978-1-4939-4901-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 295
- Number of Illustrations
- 3 b/w illustrations, 86 illustrations in colour
- Topics
