Editors:
- Includes cutting-edge methods and protocols for imaging flow cytometry
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1389)
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Table of contents (20 protocols)
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Front Matter
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IFC in Detection of Nuclei and Nucleic Acids
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Front Matter
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IFC in Immune Response and Drug Screening
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Front Matter
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IFC in Studies of Cell Death and Apoptosis
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Front Matter
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About this book
This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols willbe a critical source for all laboratories seeking to implement IFC in their research studies.
Reviews
“The purpose is to present to the scientific community the compendium of recently developed imaging flow cytometry techniques and protocols. The authors are successful in achieving these worthy objectives. Intended for the scientific community, this book will be very useful for students, graduate students, and scientists interested in learning about imaging flow cytometry and its applications. … This book for the first time explores the techniques and protocols involving quantitative imaging flow cytometry in a fascinating manner.” (Omer Iqbal, Doody's Book Reviews, September, 2016)
Editors and Affiliations
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Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, USA
Natasha S. Barteneva
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A.N. Belozersky Institute of Physico-Che, M.V. Lomonosov Moscow State University, Moscow, Russia
Ivan A. Vorobjev
Bibliographic Information
Book Title: Imaging Flow Cytometry
Book Subtitle: Methods and Protocols
Editors: Natasha S. Barteneva, Ivan A. Vorobjev
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3302-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3300-6Published: 23 November 2015
Softcover ISBN: 978-1-4939-4901-4Published: 23 August 2016
eBook ISBN: 978-1-4939-3302-0Published: 21 November 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 295
Number of Illustrations: 3 b/w illustrations, 86 illustrations in colour
Topics: Imaging / Radiology