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Recombinant Proteins from Plants

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Jacqueline MacDonald

    1. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Igor Kolotilin

    1. Department of Biology, Western University, London, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Rima Menassa

    1. London, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1385)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. Vectors and Transformation

    1. Front Matter

      Pages 1-1
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    2. Chloroplast-Based Expression of Recombinant Proteins by Gateway® Cloning Technology

      • Johanna Gottschamel, Andreas Lössl
      Pages 3-27

    3. Multigene Engineering in Rice Using High-Capacity Agrobacterium tumefaciens BIBAC Vectors

      • Ruifeng He
      Pages 29-37

    4. Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

      • Pooja Saxena, Eva C. Thuenemann, Frank Sainsbury, George P. Lomonossoff
      Pages 39-54

    5. Transient Protein Expression by Agroinfiltration in Lettuce

      • Qiang Chen, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssa McNulty, Kahlin Leuzinger et al.
      Pages 55-67

    6. Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas reinhardtii

      • Daniel Guzmán-Zapata, Karla Soledad Macedo-Osorio, Alma Lorena Almaraz-Delgado, NoĂ© Durán-Figueroa, Jesus AgustĂ­n Badillo-Corona
      Pages 69-85

  3. Product Improvements

    1. Front Matter

      Pages 87-87
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    2. Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes

      • Nicoletta Ferradini, Angelica Giancaspro, Alessandro Nicolia, Agata Gadaleta, Fabio Veronesi, Daniele Rosellini
      Pages 89-98

    3. Transient Expression of Mammalian Genes in N. benthamiana to Modulate N-Glycosylation

      • Alexandra Castilho, Herta Steinkellner
      Pages 99-113

    4. Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants

      • StĂ©phanie Robert, Philippe V. Jutras, Moustafa Khalf, Marc-AndrĂ© D’Aoust, Marie-Claire Goulet, Frank Sainsbury et al.
      Pages 115-126

  4. Extraction and Scale-Up

    1. Front Matter

      Pages 127-127
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    2. Production of Recombinant Cholera Toxin B Subunit in Nicotiana benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector

      • Lauren Moore, Krystal Hamorsky, Nobuyuki Matoba
      Pages 129-137

    3. Total Soluble Protein Extraction for Improved Proteomic Analysis of Transgenic Rice Plant Roots

      • Manish L. Raorane, Joan O. Narciso, Ajay Kohli
      Pages 139-147

    4. Temporary Immersion Bioreactors for the Contained Production of Recombinant Proteins in Transplastomic Plants

      • Sherwin Barretto, Franck Michoux, Peter J. Nixon
      Pages 149-160

    5. Plant Cell-Based Recombinant Antibody Manufacturing with a 200 L Orbitally Shaken Disposable Bioreactor

      • Nicole Raven, Stefan Schillberg, Stefan Rasche
      Pages 161-172

    6. Liquid-Liquid Phase Separation of Oil Bodies from Seeds

      • Cory L. Nykiforuk
      Pages 173-188

    7. Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture Extract

      • Lauri J. Reuter, Andrew J. Conley, Jussi J. Joensuu
      Pages 189-197

  5. Analysis

    1. Front Matter

      Pages 199-199
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    2. Molecular Analyses of Transgenic Plants

      • Kurniawan Rudi Trijatmiko, Felichi Mae Arines, Norman Oliva, Inez Hortense Slamet-Loedin, Ajay Kohli
      Pages 201-222
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Keywords

About this book

This volume provides up-to-date scientific achievements from the world’s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.

Editors and Affiliations

  • Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, Canada

    Jacqueline MacDonald

  • Department of Biology, Western University, London, Canada

    Igor Kolotilin

  • London, Canada

    Rima Menassa

Bibliographic Information

  • Book Title: Recombinant Proteins from Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Jacqueline MacDonald, Igor Kolotilin, Rima Menassa

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3289-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3288-7Published: 27 November 2015

  • Softcover ISBN: 978-1-4939-5004-1Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3289-4Published: 27 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 296

  • Number of Illustrations: 31 b/w illustrations, 33 illustrations in colour

  • Topics: Plant Sciences, Plant Biochemistry

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