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Methods in Molecular Biology

2-D PAGE Map Analysis

Methods and Protocols

Editors: Marengo, Emilio, Robotti, Elisa (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
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  • ISBN 978-1-4939-3255-9
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-3254-2
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  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
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  • ISBN 978-1-4939-4949-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Exploring the 2-D gel mapping field, the chapters in this book are separated into four different categories: Part I talks about 2-D maps reproducibility and maps modeling; Part II describes the image analysis tools that provide spot volume datasets; Part III is about the statistical methods applied to spot volume datasets to identify candidate biomarkers; and Part IV discusses differential analysis from direct image analysis tools. 2-D PAGE Map Analysis: Methods and Protocols provides a unique approach to 2-D gel mapping, in that it helps users avoid drawbacks due to ignorance of the basic theoretical mechanisms underlying the technique, including data handling and proper tools for spot analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, 2-D PAGE Map Analysis: Methods and Protocols, is a useful resource for any scientist or researcher, with a mathematical background, who is interested in 2-D gel mapping.

About the authors

Dr Elisa Robotti, PhD Dip. Scienze e Innovazione Tecnologica Università del Piemonte Orientale Viale Michel 11 15121 Alessandria Tel: +39 0131 360272 Fax: +39 0131 360250 e-mail: elisa.robotti@mfn.unipmn.it Emilio Marengo Department of Environmental and Life Sciences, University of Eastern Piedmont, Alessandria, Italy. marengoe@tin.it

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Sources of Experimental Variation in 2-D Maps: The Importance of Experimental Design in Gel-Based Proteomics

    Pages 3-37

    Valcu, Cristina-Maria (et al.)

  • Decoding 2-D Maps by Autocovariance Function

    Pages 39-53

    Pietrogrande, Maria Chiara (et al.)

  • Two-Dimensional Gel Electrophoresis Image Analysis via Dedicated Software Packages

    Pages 55-65

    Maurer, Martin H.

  • Comparative Evaluation of Software Features and Performances

    Pages 69-78

    Cecconi, Daniela

  • Image Pretreatment Tools I: Algorithms for Map Denoising and Background Subtraction Methods

    Pages 79-89

    Cannistraci, Carlo Vittorio (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3255-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-3254-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-4949-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
2-D PAGE Map Analysis
Book Subtitle
Methods and Protocols
Editors
  • Emilio Marengo
  • Elisa Robotti
Series Title
Methods in Molecular Biology
Series Volume
1384
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3255-9
DOI
10.1007/978-1-4939-3255-9
Hardcover ISBN
978-1-4939-3254-2
Softcover ISBN
978-1-4939-4949-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 331
Topics