Methods in Molecular Biology

Nucleic Acid Aptamers

Selection, Characterization, and Application

Editors: Mayer, Günter (Ed.)

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  • Includes cutting-edge methods and protocols for aptamer research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-3197-2
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About this book

This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Selection of Aptamers for Metabolite Sensing and Construction of Optical Nanosensors

    Pages 3-19

    Long, Yi (et al.)

  • SELEX of Cell-Specific RNA Aptamers

    Pages 21-32

    Berg, Katharina (et al.)

  • Developing Aptamers by Cell-Based SELEX

    Pages 33-46

    Catuogno, Silvia (et al.)

  • DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System

    Pages 47-60

    Kimoto, Michiko (et al.)

  • Capillary Electrophoresis for the Selection of DNA Aptamers Recognizing Activated Protein C

    Pages 61-75

    Hamedani, Nasim Shahidi (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3197-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-3196-5
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.99
price for USA in USD
  • ISBN 978-1-4939-4934-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Nucleic Acid Aptamers
Book Subtitle
Selection, Characterization, and Application
Editors
  • Günter Mayer
Series Title
Methods in Molecular Biology
Series Volume
1380
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3197-2
DOI
10.1007/978-1-4939-3197-2
Hardcover ISBN
978-1-4939-3196-5
Softcover ISBN
978-1-4939-4934-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 242
Number of Illustrations
20 b/w illustrations, 29 illustrations in colour
Topics