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Lipid Signaling Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Mark G. Waugh

    1. University College of London School of Life and Medical Sciences, London, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1376)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format

    • Mindy I. Davis, Atsuo T. Sasaki, Anton Simeonov
    Pages 1-9

  3. Assaying Ceramide Synthase Activity In Vitro and in Living Cells Using Liquid Chromatography-Mass Spectrometry

    • Xin Ying Lim, Russell Pickford, Anthony S. Don
    Pages 11-22

  4. Fluorescent Assays for Ceramide Synthase Activity

    • Timothy A. Couttas, Anthony S. Don
    Pages 23-33

  5. Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-Kinase Type II Alpha

    • Avanti Gokhale, Pearl V. Ryder, Stephanie A. Zlatic, Victor Faundez
    Pages 35-42

  6. Measurement of Long-Chain Fatty Acyl-CoA Synthetase Activity

    • Joachim Füllekrug, Margarete Poppelreuther
    Pages 43-53

  7. Qualitative and Quantitative In Vitro Analysis of Phosphatidylinositol Phosphatase Substrate Specificity

    • Laura Ren Huey Ip, Christina Anja Gewinner
    Pages 55-75

  8. Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity

    • Matthew C. Gage, Benoit Pourcet, Inés Pineda-Torra
    Pages 77-85

  9. Metabolically Biotinylated Reporters for Electron Microscopic Imaging of Cytoplasmic Membrane Microdomains

    • Kimberly J. Krager, John G. Koland
    Pages 87-96

  10. Fluorescence Recovery After Photobleaching Analysis of the Diffusional Mobility of Plasma Membrane Proteins: HER3 Mobility in Breast Cancer Cell Membranes

    • Mitul Sarkar, John G. Koland
    Pages 97-105

  11. Isolation and Analysis of Detergent-Resistant Membrane Fractions

    • Massimo Aureli, Sara Grassi, Sandro Sonnino, Alessandro Prinetti
    Pages 107-131

  12. Detection of Isolated Mitochondria-Associated ER Membranes Using the Sigma-1 Receptor

    • Abasha Lewis, Shang-Yi Tsai, Tsung-Ping Su
    Pages 133-140

  13. Using Surface Plasmon Resonance to Quantitatively Assess Lipid–Protein Interactions

    • Kathryn Del Vecchio, Robert V. Stahelin
    Pages 141-153

  14. Analyzing Protein–Phosphoinositide Interactions with Liposome Flotation Assays

    • Ricarda A. Busse, Andreea Scacioc, Amanda M. Schalk, Roswitha Krick, Michael Thumm, Karin Kühnel
    Pages 155-162

  15. High-Throughput Fluorometric Assay for Membrane–Protein Interaction

    • Wonhwa Cho, Hyunjin Kim, Yusi Hu
    Pages 163-174

  16. Guidelines for the Use of Protein Domains in Acidic Phospholipid Imaging

    • Matthieu Pierre Platre, Yvon Jaillais
    Pages 175-194

  17. Analysis of Sphingolipid Synthesis and Transport by Metabolic Labeling of Cultured Cells with [3H]Serine

    • Neale D. Ridgway
    Pages 195-202

  18. Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells

    • Krista Rombouts, Vinicio Carloni
    Pages 203-212

  19. Analysis of the Phosphoinositide Composition of Subcellular Membrane Fractions

    • Deborah A. Sarkes, Lucia E. Rameh
    Pages 213-227

  20. Single-Molecule Imaging of Signal Transduction via GPI-Anchored Receptors

    • Kenichi G. N. Suzuki
    Pages 229-238
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Keywords

About this book

In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

 

Editors and Affiliations

  • University College of London School of Life and Medical Sciences, London, United Kingdom

    Mark G. Waugh

Bibliographic Information

  • Book Title: Lipid Signaling Protocols

  • Editors: Mark G. Waugh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3170-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3169-9Published: 10 November 2015

  • Softcover ISBN: 978-1-4939-5007-2Published: 23 August 2016

  • eBook ISBN: 978-1-4939-3170-5Published: 09 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 257

  • Topics: Cell Biology, Membrane Biology, Lipidology

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