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Methods in Molecular Biology

Lipid Signaling Protocols

Editors: Waugh, Mark G. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-3170-5
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Hardcover $169.99
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  • ISBN 978-1-4939-3169-9
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Softcover $119.99
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  • ISBN 978-1-4939-5007-2
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About this book

In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

 

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase α in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format

    Pages 1-9

    Davis, Mindy I. (et al.)

  • Assaying Ceramide Synthase Activity In Vitro and in Living Cells Using Liquid Chromatography-Mass Spectrometry

    Pages 11-22

    Lim, Xin Ying (et al.)

  • Fluorescent Assays for Ceramide Synthase Activity

    Pages 23-33

    Couttas, Timothy A. (et al.)

  • Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-Kinase Type II Alpha

    Pages 35-42

    Gokhale, Avanti (et al.)

  • Measurement of Long-Chain Fatty Acyl-CoA Synthetase Activity

    Pages 43-53

    Füllekrug, Joachim, Ph.D. (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-3170-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-1-4939-3169-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.99
price for USA in USD
  • ISBN 978-1-4939-5007-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Lipid Signaling Protocols
Editors
  • Mark G. Waugh
Series Title
Methods in Molecular Biology
Series Volume
1376
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3170-5
DOI
10.1007/978-1-4939-3170-5
Hardcover ISBN
978-1-4939-3169-9
Softcover ISBN
978-1-4939-5007-2
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 257
Topics