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Induced Pluripotent Stem (iPS) Cells

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa Hospital Research Institute, Ottawa, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Andras Nagy

    1. Mount Sinai Hospital Samuel Lunenfeld Research Inst, Toronto, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1357)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. The piggyBac Transposon as a Platform Technology for Somatic Cell Reprogramming Studies in Mouse

    • Knut Woltjen, Shin-Il Kim, Andras Nagy
    Pages 1-22

  3. Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus

    • Filipa A. C. Soares, Roger A. Pedersen, Ludovic Vallier
    Pages 23-31

  4. Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton’s Jelly into Induced Pluripotent Stem Cells

    • Cristian Miere, Liani Devito, Dusko Ilic
    Pages 33-44

  5. Very Rapid and Efficient Generation of Induced Pluripotent Stem Cells from Mouse Pre-B Cells

    • Bruno Di Stefano, Thomas Graf
    Pages 45-56

  6. Generation of iPS Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

    • Ruijun Jeanna Su, Amanda Neises, Xiao-Bing Zhang
    Pages 57-69

  7. Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA

    • Jason Dang, Tariq M Rana
    Pages 71-84

  8. Generation of Partially Reprogrammed Cells and Fully Reprogrammed iPS Cells by Plasmid Transfection

    • Jong Soo Kim, Hyun Woo Choi, Yean Ju Hong, Jeong Tae Do
    Pages 85-95

  9. Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs)

    • Lucas V. Greder, Jason Post, James R. Dutton
    Pages 97-110

  10. Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons

    • Shin-Il Kim, Fabian Oceguera-Yanez, Chiho Sakurai, Masato Nakagawa, Shinya Yamanaka, Knut Woltjen
    Pages 111-131

  11. Generation and Characterization of Rat iPSCs

    • Jing Liao, Chun Cui
    Pages 133-148

  12. Generation of Induced Pluripotent Stem Cells in Rabbits

    • Marielle Afanassieff, Yann Tapponnier, Pierre Savatier
    Pages 149-172

  13. Cynomolgus Monkey Induced Pluripotent Stem Cells Generated By Using Allogeneic Genes

    • Nobuhiro Shimozawa
    Pages 173-182

  14. Induced Pluripotent Stem Cells from Nonhuman Primates

    • Anuja Mishra, Zhifang Qiu, Steven L. Farnsworth, Jacob J. Hemmi, Miao Li, Alexander V. Pickering et al.
    Pages 183-193

  15. Computational Biology Methods for Characterization of Pluripotent Cells

    • Marcos J. Araúzo-Bravo
    Pages 195-220

  16. cGMP-Compliant Expansion of Human iPSC Cultures as Adherent Monolayers

    • Ann M. Parr, Patrick J. Walsh, Vincent Truong, James R. Dutton
    Pages 221-229

  17. Analysis of the Mitochondrial DNA and Its Replicative Capacity in Induced Pluripotent Stem Cells

    • Gael Cagnone, Vijesh Vaghjiani, William Lee, Claire Sun, Jacqueline Johnson, Ka-Yu Yeung et al.
    Pages 231-267

  18. Selection of Phage Display Peptides Targeting Human Pluripotent Stem Cell-Derived Progenitor Cell Lines

    • Paola A. Bignone, Rachel A. Krupa, Michael D. West, David Larocca
    Pages 269-283

  19. piggyBac Transposon Mediated Reprogramming and Flow Cytometry Analysis of CD44 and ICAM1 Cell-Surface Marker Changes

    • Sara Brightwell, Keisuke Kaji
    Pages 285-293

  20. Generation and In Vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells

    • Ayaka Yanagida, Hiromitsu Nakauchi, Akihide Kamiya
    Pages 295-310
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Keywords

About this book

This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocols guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols provides protocols that can be used by both experts and novices interested in stem cells.

 

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

  • Mount Sinai Hospital Samuel Lunenfeld Research Inst, Toronto, Canada

    Andras Nagy

Bibliographic Information

  • Book Title: Induced Pluripotent Stem (iPS) Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen, Andras Nagy

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3055-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3054-8Published: 29 December 2015

  • Softcover ISBN: 978-1-4939-7981-3Published: 27 March 2019

  • eBook ISBN: 978-1-4939-3055-5Published: 28 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 496

  • Number of Illustrations: 32 b/w illustrations, 44 illustrations in colour

  • Topics: Cell Biology

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