Methods in Molecular Biology

Phospho-Proteomics

Methods and Protocols

Editors: von Stechow, Louise (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-3049-4
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About this book

This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phosphoproteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Phosphoproteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • Thiol-ene-Enabled Detection of Thiophosphorylation as a Labeling Strategy for Phosphoproteins

    Pages 3-15

    Wilke, Kaelyn E. (et al.)

  • Phosphopeptide Detection with Biotin-Labeled Phos-tag

    Pages 17-29

    Kinoshita-Kikuta, Emiko (et al.)

  • Phosphopeptide Enrichment by Covalent Chromatography After Solid Phase Derivatization of Protein Digests on Reversed Phase Supports

    Pages 31-50

    Nika, Heinz (et al.)

  • Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics

    Pages 53-70

    Cheng, Lei (et al.)

  • Identification of Direct Kinase Substrates Using Analogue-Sensitive Alleles

    Pages 71-84

    Rothenberg, Daniel A. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-3049-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
  • Institutional customers should get in touch with their account manager
Hardcover $179.99
price for USA in USD
Softcover $139.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Phospho-Proteomics
Book Subtitle
Methods and Protocols
Editors
  • Louise von Stechow
Series Title
Methods in Molecular Biology
Series Volume
1355
Copyright
2016
Publisher
Springer-Verlag New York
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-3049-4
DOI
10.1007/978-1-4939-3049-4
Hardcover ISBN
978-1-4939-3048-7
Softcover ISBN
978-1-4939-5001-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIII, 364
Topics