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Methods in Molecular Biology

Single Cell Protein Analysis

Methods and Protocols

Editors: Singh, Anup K., Chandrasekaran, Aarthi (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-2987-0
  • Digitally watermarked, DRM-free
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  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $119.99
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About this book

This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells. 

About the authors

Anup Singh Mailstop 9291 Sandia National Laboratories 7011 East Ave Livermore, CA aksingh@sandia.gov

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Single-Cell Western Blotting

    Pages 1-9

    Sinkala, Elly (et al.)

  • A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. coli Bacteria

    Pages 11-25

    Stratz, Simone (et al.)

  • Enzyme-Linked ImmunoSpot (ELISpot) for Single-Cell Analysis

    Pages 27-46

    Janetzki, Sylvia (et al.)

  • Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells

    Pages 47-54

    Ullal, Adeeti V. (et al.)

  • Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia coli Cells with Single-Molecule Sensitivity

    Pages 55-67

    Taniguchi, Yuichi

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-2987-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $119.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Single Cell Protein Analysis
Book Subtitle
Methods and Protocols
Editors
  • Anup K. Singh
  • Aarthi Chandrasekaran
Series Title
Methods in Molecular Biology
Series Volume
1346
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2987-0
DOI
10.1007/978-1-4939-2987-0
Hardcover ISBN
978-1-4939-2986-3
Softcover ISBN
978-1-4939-4943-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 254
Topics