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Single Cell Protein Analysis

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Anup K. Singh

    1. Sandia National Laboratories , Livermore, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Aarthi Chandrasekaran

    1. Sandia National Laboratories, Livermore, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1346)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Single-Cell Western Blotting

    • Elly Sinkala, Amy E. Herr
    Pages 1-9

  3. A Microfluidic Device for Immunoassay-Based Protein Analysis of Single E. coli Bacteria

    • Simone Stratz, Petra S. Dittrich
    Pages 11-25

  4. Enzyme-Linked ImmunoSpot (ELISpot) for Single-Cell Analysis

    • Sylvia Janetzki, Rachel Rabin
    Pages 27-46

  5. Photocleavable DNA Barcoding Antibodies for Multiplexed Protein Analysis in Single Cells

    • Adeeti V. Ullal, Ralph Weissleder
    Pages 47-54

  6. Genome-Wide Analysis of Protein and mRNA Copy Numbers in Single Escherichia coli Cells with Single-Molecule Sensitivity

    • Yuichi Taniguchi
    Pages 55-67

  7. Microfluidic Flow Cytometry for Single-Cell Protein Analysis

    • Meiye Wu, Anup K. Singh
    Pages 69-83

  8. Microfluidic Image Cytometry for Single-Cell Phenotyping of Human Pluripotent Stem Cells

    • Yasumasa Mashimo, Ken-ichiro Kamei
    Pages 85-98

  9. Characterizing Phenotypes and Signaling Networks of Single Human Cells by Mass Cytometry

    • Nalin Leelatian, Kirsten E. Diggins, Jonathan M. Irish
    Pages 99-113

  10. Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry

    • Mei Ling Leong, Evan W. Newell
    Pages 115-131

  11. Imaging and Mapping of Tissue Constituents at the Single-Cell Level Using MALDI MSI and Quantitative Laser Scanning Cytometry

    • Catherine M. Rawlins, Joseph P. Salisbury, Daniel R. Feldman, Sinan Isim, Nathalie Y. R. Agar, Ed Luther et al.
    Pages 133-149

  12. SPLIFF: A Single-Cell Method to Map Protein-Protein Interactions in Time and Space

    • Alexander Dünkler, Reinhild Rösler, Hans A. Kestler, Daniel Moreno-Andrés, Nils Johnsson
    Pages 151-168

  13. Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution

    • Matthias Blazek, Günter Roth, Roland Zengerle, Matthias Meier
    Pages 169-184

  14. Dynamics and Interactions of Individual Proteins in the Membrane of Single, Living Cells

    • Stephen Anthony, Amanda Carroll-Portillo, Jerilyn Timlin
    Pages 185-207

  15. Microfluidics-Enabled Enzyme Activity Measurement in Single Cells

    • Cinzia Tesauro, Rikke Frøhlich, Magnus Stougaard, Yi-Ping Ho, Birgitta R. Knudsen
    Pages 209-219

  16. Microfluidic Chemical Cytometry for Enzyme Assays of Single Cells

    • Livia Shehaj, Lorena Lazo de la Vega, Michelle L. Kovarik
    Pages 221-238

  17. Quantitative Detection of Nucleocytoplasmic Transport of Native Proteins in Single Cells

    • Zhenning Cao, Chang Lu
    Pages 239-252

  18. Back Matter

    Pages 253-254
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Keywords

About this book

This volume highlights recent developments in flow cytometry, affinity assays, imaging, mass spectrometry, microfluidics and other technologies that enable analysis of proteins at the single cell level. The book also includes chapters covering a suite of biochemical and biophysical methods capable of making an entire gamut of proteomic measurements, including analysis of protein abundance or expression, protein interaction networks, post-translational modifications, translocation and enzymatic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Single Cell Protein Analysis: Methods and Protocols is useful to researchers and students in biological and biomedical sciences who have an interest in proteomic measurements in cells. 

Editors and Affiliations

  • Sandia National Laboratories , Livermore, USA

    Anup K. Singh

  • Sandia National Laboratories, Livermore, USA

    Aarthi Chandrasekaran

About the editors

Anup Singh Mailstop 9291 Sandia National Laboratories 7011 East Ave Livermore, CA aksingh@sandia.gov

Bibliographic Information

  • Book Title: Single Cell Protein Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Anup K. Singh, Aarthi Chandrasekaran

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2987-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2986-3Published: 07 November 2015

  • Softcover ISBN: 978-1-4939-4943-4Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2987-0Published: 05 November 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 254

  • Topics: Protein Science

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