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TGF-β Signaling

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Xin-Hua Feng

    1. Departments of Surgery and Molecular & Cellular Biology, Baylor College of Medicine, Houston, USA

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  2. Pinglong Xu

    1. Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang Proviince, China

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    You can also search for this editor in PubMed   Google Scholar

  3. Xia Lin

    1. Department of Surgery, Baylor College of Medicine, Houston, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1344)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Regulation of TGF-β Receptors

    • Erine H. Budi, Jian Xu, Rik Derynck
    Pages 1-33

  3. Determining TGF-β Receptor Levels in the Cell Membrane

    • Long Zhang, Fangfang Zhou, Maarten van Dinther, Peter ten Dijke
    Pages 35-47

  4. Posttranslational Modifications of TGF-β Receptors

    • Xiaohua Yan, Ye-Guang Chen
    Pages 49-61

  5. Production, Isolation, and Structural Analysis of Ligands and Receptors of the TGF-β Superfamily

    • Tao Huang, Andrew P. Hinck
    Pages 63-92

  6. Phosphorylation of Smads by Intracellular Kinases

    • Fang Liu, Isao Matsuura
    Pages 93-109

  7. Analysis of Smad Phosphatase Activity In Vitro

    • Tao Shen, Lan Qin, Xia Lin
    Pages 111-119

  8. Three-dimensional Mammary Epithelial Cell Morphogenesis Model for Analysis of TGFß Signaling

    • Juliet Rashidian, Kunxin Luo
    Pages 121-135

  9. TGF-β Signaling in Stem Cell Regulation

    • Wenlin Li, Wanguo Wei, Sheng Ding
    Pages 137-145

  10. Analysis of Epithelial–Mesenchymal Transition Induced by Transforming Growth Factor β

    • Ulrich Valcourt, Jonathon Carthy, Yukari Okita, Lindsay Alcaraz, Mitsuyasu Kato, Sylvie Thuault et al.
    Pages 147-181

  11. In Vitro Th Differentiation Protocol

    • Takashi Sekiya, Akihiko Yoshimura
    Pages 183-191

  12. Interrogating TGF-β Function and Regulation in Endothelial Cells

    • J. A. Maring, L. A. van Meeteren, M. J. Goumans, Peter ten Dijke
    Pages 193-203

  13. Isolation and Manipulation of Adipogenic Cells to Assess TGF-β Superfamily Functions

    • Maria Namwanje, Juan C. Bournat, Chester W. Brown
    Pages 205-217

  14. Imaging TGFβ Signaling in Mouse Models of Cancer Metastasis

    • Yibin Kang
    Pages 219-232

  15. Generation and Characterization of Smad7 Conditional Knockout Mice

    • Yi Pan, Yan Chen
    Pages 233-243

  16. Monitoring Smad Activity In Vivo Using the Xenopus Model System

    • Marco Montagner, Graziano Martello, Stefano Piccolo
    Pages 245-259

  17. Animal Cap Assay for TGF-β Signaling

    • Chenbei Chang
    Pages 261-274

  18. Detection of Smad Signaling in Zebrafish Embryos

    • Xingfeng Liu, Qiang Wang, Anming Meng
    Pages 275-286

  19. Role of TGF-β Signaling in Coupling Bone Remodeling

    • Janet L. Crane, Lingling Xian, Xu Cao
    Pages 287-300

  20. Studying the Functions of TGF-β Signaling in the Ovary

    • Chao Yu, Jian-Jie Zhou, Heng-Yu Fan
    Pages 301-311
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Keywords

About this book

This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF-β from the basic principles to the practical applications. Chapters provide basic introduction of TGF-β signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-β signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Departments of Surgery and Molecular & Cellular Biology, Baylor College of Medicine, Houston, USA

    Xin-Hua Feng

  • Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang Proviince, China

    Pinglong Xu

  • Department of Surgery, Baylor College of Medicine, Houston, USA

    Xia Lin

Bibliographic Information

  • Book Title: TGF-β Signaling

  • Book Subtitle: Methods and Protocols

  • Editors: Xin-Hua Feng, Pinglong Xu, Xia Lin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2966-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-2965-8Published: 05 November 2015

  • Softcover ISBN: 978-1-4939-4993-9Published: 23 August 2016

  • eBook ISBN: 978-1-4939-2966-5Published: 31 October 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 394

  • Topics: Cell Biology

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