Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1344)
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Table of contents(25 protocols)
About this book
This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF-β from the basic principles to the practical applications. Chapters provide basic introduction of TGF-β signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-β signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Editors and Affiliations
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Departments of Surgery and Molecular & Cellular Biology, Baylor College of Medicine, Houston, USA
Xin-Hua Feng
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Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang Proviince, China
Pinglong Xu
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Department of Surgery, Baylor College of Medicine, Houston, USA
Xia Lin
Bibliographic Information
Book Title: TGF-β Signaling
Book Subtitle: Methods and Protocols
Editors: Xin-Hua Feng, Pinglong Xu, Xia Lin
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2966-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-2965-8Published: 05 November 2015
Softcover ISBN: 978-1-4939-4993-9Published: 23 August 2016
eBook ISBN: 978-1-4939-2966-5Published: 31 October 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 394
Topics: Cell Biology