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Cell Cycle Oscillators

Methods and Protocols

  • Book
  • © 2016

Overview

Editors:
  1. Amanda S. Coutts

    1. Department of Oncology, University of Oxford, Oxford, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Louise Weston

    1. Scientific writer, London, United Kingdom

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1342)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  3. Methods

    1. Front Matter

      Pages 89-89
      Download chapter PDF

    2. Cell Synchronization of Mouse Embryonic Fibroblasts

      • Michael J. Thwaites, Courtney H. Coschi, Christian E. Isaac, Frederick A. Dick
      Pages 91-99

    3. Cell Cycle Synchronization in Xenopus Egg Extracts

      • Peter J. Gillespie, Julia Neusiedler, Kevin Creavin, Gaganmeet Singh Chadha, J. Julian Blow
      Pages 101-147

    4. Elutriation for Cell Cycle Synchronization in Fission Yeast

      • Kazunori Kume
      Pages 149-155

    5. Spatiotemporal Investigation of Phosphorylation Events During Cell Cycle Progression

      • Lilia Gheghiani, Olivier Gavet
      Pages 157-171

    6. Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence

      • Karen Akopyan, Arne Lindqvist, Erik Müllers
      Pages 173-183

    7. Building a Synthetic Transcriptional Oscillator

      • Matthaeus Schwarz-Schilling, Jongmin Kim, Christian Cuba, Maximilian Weitz, Elisa Franco, Friedrich C. Simmel
      Pages 185-199

    8. The Use of SNAP Labeling to Study Cell Cycle Oscillatory Proteins

      • Christine Greil, Marie Follo, Monika Engelhardt, Ralph Wäsch
      Pages 201-208

    9. A Computational Method for Identifying Yeast Cell Cycle Transcription Factors

      • Wei-Sheng Wu
      Pages 209-219

    10. Measuring Activity and Specificity of Protein Phosphatases

      • Brendan L. Powers, Michael Melesse, Christie L. Eissler, Harry Charbonneau, Mark C. Hall
      Pages 221-235

    11. Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an In Vitro Cdk1 Targeting Assay to Study the Regulation of the γ-Tubulin Complex

      • Po Hien Ear, Jacqueline Kowarzyk, Michael J. Booth, Diala Abd-Rabbo, Kristian Shulist, Conrad Hall et al.
      Pages 237-257

    12. Cell Cycle Synchronization Using a Microfluidic Synchronizer for Fission Yeast Cells

      • Shujing Wang, Chunxiong Luo
      Pages 259-268

    13. Detection of Protein–Protein Interactions in Tobacco BY-2 Cells Using Bimolecular Fluorescence Complementation

      • Gemma S. Puts, Natasha Spadafora
      Pages 269-277

    14. Tracking the Cyclin B1-GFP Sensor to Profile the Pattern of Mitosis Versus Mitotic Bypass

      • Victoria Griesdoorn, M. Rowan Brown, Marie Wiltshire, Paul J. Smith, Rachel J. Errington
      Pages 279-285
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Keywords

About this book

​ This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.

Editors and Affiliations

  • Department of Oncology, University of Oxford, Oxford, United Kingdom

    Amanda S. Coutts

  • Scientific writer, London, United Kingdom

    Louise Weston

Bibliographic Information

  • Book Title: Cell Cycle Oscillators

  • Book Subtitle: Methods and Protocols

  • Editors: Amanda S. Coutts, Louise Weston

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2957-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-2956-6Published: 09 August 2015

  • Softcover ISBN: 978-1-4939-4958-8Published: 01 November 2016

  • eBook ISBN: 978-1-4939-2957-3Published: 08 August 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 352

  • Number of Illustrations: 20 b/w illustrations, 60 illustrations in colour

  • Topics: Cell Biology, Cell Cycle Analysis

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