Methods in Molecular Biology

Protein Arginylation

Methods and Protocols

Editors: Kashina, Anna S. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-2935-1
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Hardcover $159.99
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Softcover $119.00
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About this book

This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. This book also includes essays from the founders of the field, who originally discovered arginylation in the early 1960s and brought it to international recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein Arginylation: Methods and Protocols would interest the emerging body of scientists involved in the studies of posttranslational arginylation and the rapidly growing community of researchers working on a broad range of posttranslational modifications, the analysis of which often meets similar challenges and utilizes similar principles as posttranslational arginylation.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Protein Arginylation: Over 50 Years of Discovery

    Pages 1-11

    Kashina, Anna S.

  • Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase

    Pages 13-18

    Kaji, Hideko (et al.)

  • Arginyltransferase: A Personal and Historical Perspective

    Pages 19-23

    Soffer, Richard L.

  • Arginylation in a Partially Purified Fraction of 150k × g Supernatants of Axoplasm and Injured Vertebrate Nerves

    Pages 25-32

    Ingoglia, Nicholas A.

  • Preparation of ATE1 Enzyme from Native Mammalian Tissues

    Pages 33-37

    Kashina, Anna S.

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2935-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-4939-2934-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-4568-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Arginylation
Book Subtitle
Methods and Protocols
Editors
  • Anna S. Kashina
Series Title
Methods in Molecular Biology
Series Volume
1337
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2935-1
DOI
10.1007/978-1-4939-2935-1
Hardcover ISBN
978-1-4939-2934-4
Softcover ISBN
978-1-4939-4568-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
IX, 149
Number of Illustrations
8 b/w illustrations, 11 illustrations in colour
Topics