Skip to main content
SpringerLink
Log in
Book cover

Protein Arginylation

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Anna S. Kashina

    1. Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1337)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-ix
    Download chapter PDF

  2. Protein Arginylation: Over 50 Years of Discovery

    • Anna S. Kashina
    Pages 1-11

  3. Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase

    • Hideko Kaji, Akira Kaji
    Pages 13-18

  4. Arginyltransferase: A Personal and Historical Perspective

    • Richard L. Soffer
    Pages 19-23

  5. Arginylation in a Partially Purified Fraction of 150k × g Supernatants of Axoplasm and Injured Vertebrate Nerves

    • Nicholas A. Ingoglia
    Pages 25-32

  6. Preparation of ATE1 Enzyme from Native Mammalian Tissues

    • Anna S. Kashina
    Pages 33-37

  7. Correlated Measurement of Endogenous ATE1 Activity on Native Acceptor Proteins in Tissues and Cultured Cells to Detect Cellular Aging

    • Hideko Kaji, Akira Kaji
    Pages 39-48

  8. Assaying the Posttranslational Arginylation of Proteins in Cultured Cells

    • Mauricio R. Galiano, Marta E. Hallak
    Pages 49-58

  9. Assaying ATE1 Activity in Yeast by β-Gal Degradation

    • Anna S. Kashina
    Pages 59-65

  10. Bacterial Expression and Purification of Recombinant Arginyltransferase (ATE1) and Arg-tRNA Synthetase (RRS) for Arginylation Assays

    • Junling Wang, Anna S. Kashina
    Pages 67-71

  11. Assaying ATE1 Activity In Vitro

    • Junling Wang, Anna S. Kashina
    Pages 73-77

  12. High-Throughput Arginylation Assay in Microplate Format

    • Sougata Saha, Junling Wang, Anna S. Kashina
    Pages 79-82

  13. Assay of Arginyltransferase Activity by a Fluorescent HPLC Method

    • Koichi Takao
    Pages 83-91

  14. Identification of Arginylated Proteins by Mass Spectrometry

    • Anna S. Kashina, John R. Yates III
    Pages 93-104

  15. Analysis of Arginylated Peptides by Subtractive Edman Degradation

    • Anna S. Kashina, John R. Yates III
    Pages 105-107

  16. Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles

    • Anne M. Wagner, John B. Warner, Haviva E. Garrett, Christopher R. Walters, E. James Petersson
    Pages 109-127

  17. Applying Arginylation for Bottom-Up Proteomics

    • H. Alexander Ebhardt
    Pages 129-138

  18. Development of New Tools for the Studies of Protein Arginylation

    • Anna S. Kashina
    Pages 139-145

  19. Back Matter

    Pages 147-149
    Download chapter PDF
Back to top

Keywords

About this book

This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. This book also includes essays from the founders of the field, who originally discovered arginylation in the early 1960s and brought it to international recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Protein Arginylation: Methods and Protocols would interest the emerging body of scientists involved in the studies of posttranslational arginylation and the rapidly growing community of researchers working on a broad range of posttranslational modifications, the analysis of which often meets similar challenges and utilizes similar principles as posttranslational arginylation.

Editors and Affiliations

  • Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, USA

    Anna S. Kashina

Bibliographic Information

  • Book Title: Protein Arginylation

  • Book Subtitle: Methods and Protocols

  • Editors: Anna S. Kashina

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2935-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2934-4Published: 20 August 2015

  • Softcover ISBN: 978-1-4939-4568-9Published: 22 October 2016

  • eBook ISBN: 978-1-4939-2935-1Published: 18 August 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: IX, 149

  • Number of Illustrations: 8 b/w illustrations, 11 illustrations in colour

  • Topics: Protein Science

Publish with us

Policies and ethics

Back to top

Search

Navigation