- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This volume presents a comprehensive overview of all the existing methods on analysing protein arginylation, from the early methods utilizing crude protein preparations and whole-cell assays to the latest advanced methods involving recombinant protein techniques, antibodies, high precision mass spectrometry, and chemical probes. This book also includes essays from the founders of the field, who originally discovered arginylation in the early 1960s and brought it to international recognition. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Protein Arginylation: Methods and Protocols would interest the emerging body of scientists involved in the studies of posttranslational arginylation and the rapidly growing community of researchers working on a broad range of posttranslational modifications, the analysis of which often meets similar challenges and utilizes similar principles as posttranslational arginylation.
- Table of contents (17 chapters)
-
-
Protein Arginylation: Over 50 Years of Discovery
Pages 1-11
-
Recollection of How We Came Across the Protein Modification with Amino Acids by Aminoacyl tRNA-Protein Transferase
Pages 13-18
-
Arginyltransferase: A Personal and Historical Perspective
Pages 19-23
-
Arginylation in a Partially Purified Fraction of 150k × g Supernatants of Axoplasm and Injured Vertebrate Nerves
Pages 25-32
-
Preparation of ATE1 Enzyme from Native Mammalian Tissues
Pages 33-37
-
Table of contents (17 chapters)
Recommended for you
Bibliographic Information
- Bibliographic Information
-
- Book Title
- Protein Arginylation
- Book Subtitle
- Methods and Protocols
- Editors
-
- Anna S. Kashina
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1337
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2935-1
- DOI
- 10.1007/978-1-4939-2935-1
- Hardcover ISBN
- 978-1-4939-2934-4
- Softcover ISBN
- 978-1-4939-4568-9
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- IX, 149
- Number of Illustrations
- 8 b/w illustrations, 11 illustrations in colour
- Topics
