Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1336)
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Table of contents (16 protocols)
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Front Matter
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Back Matter
About this book
This volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book describes elaborate procedures to evaluate activity and mechanism of action of new and already identified CDK inhibitors. The third part of the book talks about protocols to evaluate metabolomics changes associated to inhibitor treatment. Drug delivery strategies focused on nanoparticle development to provide alternative internalization systems for increasing inhibitor efficacy are also described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Cyclin-Dependent Kinase (CDK) Inhibitors: Methods and Protocols is a useful tool for scientists interested in this research field.
Editors and Affiliations
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Medicinal Chemistry, Centro de Investigación Príncipe Felipe, Valencia, Spain
Mar Orzáez, Mónica Sancho Medina
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IBV-CSIC and Centro de Investigación Príncipe Felipe, Valencia, Spain
Enrique Pérez-Payá
Bibliographic Information
Book Title: Cyclin-Dependent Kinase (CDK) Inhibitors
Book Subtitle: Methods and Protocols
Editors: Mar Orzáez, Mónica Sancho Medina, Enrique Pérez-Payá
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2926-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2016
Hardcover ISBN: 978-1-4939-2925-2Published: 05 August 2015
Softcover ISBN: 978-1-4939-4368-5Published: 19 October 2016
eBook ISBN: 978-1-4939-2926-9Published: 31 July 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 227
Number of Illustrations: 20 b/w illustrations, 22 illustrations in colour
Topics: Pharmacology/Toxicology