Methods in Molecular Biology

VEGF Signaling

Methods and Protocols

Editors: Fiedler, Lorna (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-2917-7
  • Digitally watermarked, DRM-free
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Hardcover $169.99
price for USA in USD
Softcover $119.99
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About this book

This volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clinical investigations. Each of the three sections begins with an introductory overview followed by supporting relevant methods. A major advantage of this book is that each chapter not only contains methodological detail rarely seen in other literature, but also contains a section on key notes and troubleshooting advice. Part I focuses on quantification of specific VEGF and VEGF receptor isoforms, beginning with a summary on VEGF splice variants, their function and their regulation. Part II relates to the study of VEGF dependent signaling in vitro, starting with a comprehensive overview of VEGF-mediated signal transduction in the cardiovascular system that highlights recent discoveries in the field. Part III first details genetic mouse models, then discusses techniques for studying physiological angiogenesis in the developing mouse embryo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, VEFG Signaling: Methods and Protocols is a useful tool for researching and understanding the basic biology of VEGF signaling and translating it into the clinic.

About the authors

Lorna R. Fielder, Program in cardiovascular and Metabolic Disorders, Duke-NUS, Graduate Medical School Singapore, 8 College Road, Singapore 169857, Telephone: +65-6516-7666, Email: lorna.fiedler@gmail.com or l.fiedler@imperial.ac.uk.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • VEGF Splicing and the Role of VEGF Splice Variants: From Physiological-Pathological Conditions to Specific Pre-mRNA Splicing

    Pages 3-23

    Guyot, Mélanie (et al.)

  • Detection and Quantification of VEGF Isoforms by ELISA

    Pages 25-37

    Vernes, Jean-Michel (et al.)

  • Quantitation of Circulating Neuropilin-1 in Human, Monkey, Mouse, and Rat Sera by ELISA

    Pages 39-48

    Lu, Yanmei (et al.)

  • Detection and Quantification of Vascular Endothelial Growth Factor Receptor Tyrosine Kinases in Primary Human Endothelial Cells

    Pages 49-65

    Fearnley, Gareth W. (et al.)

  • Induction of VEGF Secretion in Cardiomyocytes by Mechanical Stretch

    Pages 67-74

    Matter, Michelle L., Ph.D.

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-2917-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $119.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
VEGF Signaling
Book Subtitle
Methods and Protocols
Editors
  • Lorna Fiedler
Series Title
Methods in Molecular Biology
Series Volume
1332
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2917-7
DOI
10.1007/978-1-4939-2917-7
Hardcover ISBN
978-1-4939-2916-0
Softcover ISBN
978-1-4939-4370-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 225
Number of Illustrations
13 b/w illustrations, 23 illustrations in colour
Topics