Methods in Molecular Biology

The BAM Complex

Methods and Protocols

Editors: Buchanan, Susan K., Noinaj, Nicholas (Eds.)

Free Preview
  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-2871-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $179.99
price for USA in USD
Softcover $119.00
price for USA in USD
About this book

This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • The β-Barrel Assembly Machinery Complex

    Pages 1-16

    Leyton, Denisse L. (et al.)

  • Yeast Mitochondria as a Model System to Study the Biogenesis of Bacterial β-Barrel Proteins

    Pages 17-31

    Ulrich, Thomas (et al.)

  • Experimental Methods for Studying the BAM Complex in Neisseria meningitidis

    Pages 33-49

    Bos, Martine P. (et al.)

  • Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria

    Pages 51-56

    Noinaj, Nicholas (et al.)

  • The Role of a Destabilized Membrane for OMP Insertion

    Pages 57-65

    Plummer, Ashlee M. (et al.)

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-2871-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $179.99
price for USA in USD
Softcover $119.00
price for USA in USD
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
The BAM Complex
Book Subtitle
Methods and Protocols
Editors
  • Susan K. Buchanan
  • Nicholas Noinaj
Series Title
Methods in Molecular Biology
Series Volume
1329
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2871-2
DOI
10.1007/978-1-4939-2871-2
Hardcover ISBN
978-1-4939-2870-5
Softcover ISBN
978-1-4939-4920-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 283
Number of Illustrations
27 b/w illustrations, 16 illustrations in colour
Topics