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Methods in Molecular Biology

Hedgehog Signaling Protocols

Editors: Riobo, Natalia A. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-2772-2
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Hardcover $119.00
price for USA in USD
  • ISBN 978-1-4939-2771-5
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  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
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  • ISBN 978-1-4939-4922-9
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About this book

The second edition of this volume provides novel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and mathematical models for studying Hh proteins gradient formation in tissues. The next few chapters explore studies in cell culture using fibroblasts that respond readily to Hh proteins. The book goes on to present protocols for evaluation of transcriptional activity of the Gli transcription factors in cell cultures and determine the expression level of endogenous Gli proteins. The final chapters talk about metabolic effects of noncanonical Hh signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Hedgehog Signaling Protocols, Second Edition presents researchers with a comprehensive understanding of the Hh pathway and its function in development and diseases.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate

    Pages 1-7

    Johnson, Noah Ray (et al.)

  • In Vivo Imaging of Hedgehog Transport in Drosophila Epithelia

    Pages 9-18

    Seijo-Barandiarán, Irene (et al.)

  • Modeling Hedgehog Signaling Through Flux-Saturated Mechanisms

    Pages 19-33

    Sánchez, Óscar (et al.)

  • Evaluating the Activity of Smoothened Toward G Proteins Using [35S]Guanosine 5′-(3-O-thio)triphosphate ([35S]GTPγS)

    Pages 35-44

    Manning, David R. (et al.)

  • Analysis of Smoothened Phosphorylation and Activation in Cultured Cells and Wing Discs of Drosophila

    Pages 45-60

    Jiang, Kai (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2772-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA in USD
  • ISBN 978-1-4939-2771-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-4922-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Hedgehog Signaling Protocols
Editors
  • Natalia A. Riobo
Series Title
Methods in Molecular Biology
Series Volume
1322
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2772-2
DOI
10.1007/978-1-4939-2772-2
Hardcover ISBN
978-1-4939-2771-5
Softcover ISBN
978-1-4939-4922-9
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 200
Number of Illustrations
14 b/w illustrations, 28 illustrations in colour
Topics