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Methods in Molecular Biology

Nucleic Acid Crystallography

Methods and Protocols

Editors: Ennifar, Eric (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-4939-2763-0
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Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-2762-3
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Softcover $149.99
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  • ISBN 978-1-4939-4641-9
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About this book

This volume includes comprehensive and up-to-date coverage of all nucleic-acid-specific steps used in X-ray crystallography, from macromolecule production to structure determination. Chapters dedicated to RNA preparation and crystallogenesis will be of interest to beginners, while chapters focused on data collection, phasing and refinement will be particularly useful to researchers with a higher level of expertise. Several functional case studies are also presented in the last part of the book. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Nucleic Acid Crystallography: Methods and Protocols presents protocols that are aimed at both researchers and students who are interested in the structural biology of DNA or RNA, alone or in complex with proteins or ligands.

About the authors

Eric Ennifar Institut de Biologie Moléculaire et Cellulaire du CNRS 15 Rue René Descartes F-67084 Strasbourg, France +33 (0)3 88 41 70 02 email: e.ennifar@ibmc-cnrs.unistra.fr.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Perspectives and Pitfalls in Nucleic Acids Crystallography

    Pages 3-8

    Westhof, Eric

  • Preparation of Short 5′-Triphosphorylated Oligoribonucleotides for Crystallographic and Biochemical Studies

    Pages 11-20

    Vasilyev, Nikita (et al.)

  • Preparation and Crystallization of Riboswitches

    Pages 21-36

    Peselis, Alla (et al.)

  • In Vitro/In Vivo Production of tRNA for X-Ray Studies

    Pages 37-57

    Dégut, Clément (et al.)

  • Polyacrylamide Gel Electrophoresis for Purification of Large Amounts of RNA

    Pages 59-65

    Meyer, Mélanie (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2763-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-2762-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.99
price for USA in USD
  • ISBN 978-1-4939-4641-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Nucleic Acid Crystallography
Book Subtitle
Methods and Protocols
Editors
  • Eric Ennifar
Series Title
Methods in Molecular Biology
Series Volume
1320
Copyright
2016
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2763-0
DOI
10.1007/978-1-4939-2763-0
Hardcover ISBN
978-1-4939-2762-3
Softcover ISBN
978-1-4939-4641-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 356
Number of Illustrations
18 b/w illustrations, 98 illustrations in colour
Topics