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Methods in Molecular Biology

Glyco-Engineering

Methods and Protocols

Editors: Castilho, Alexandra (Ed.)

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  • Features protocols in glyco-engineering in bacteria, yeast, insects, plants and mammalian cells
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2760-9
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  • ebooks can be used on all reading devices
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-2759-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-4942-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.

Table of contents (29 chapters)

Table of contents (29 chapters)
  • Current Approaches to Engineering N-Linked Protein Glycosylation in Bacteria

    Pages 3-16

    Naegeli, Andreas (et al.)

  • Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli

    Pages 17-35

    Jaffé, Stephen R. P. (et al.)

  • GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes

    Pages 37-47

    Ollis, Anne A. (et al.)

  • Production of Glycoproteins with Asparagine-Linked N-Acetylglucosamine in Escherichia coli

    Pages 49-56

    Schwarz, Flavio (et al.)

  • Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in E. coli

    Pages 57-70

    Vozza, Nicolas F. (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2760-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-2759-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-4942-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Glyco-Engineering
Book Subtitle
Methods and Protocols
Editors
  • Alexandra Castilho
Series Title
Methods in Molecular Biology
Series Volume
1321
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2760-9
DOI
10.1007/978-1-4939-2760-9
Hardcover ISBN
978-1-4939-2759-3
Softcover ISBN
978-1-4939-4942-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 439
Number of Illustrations
27 b/w illustrations, 52 illustrations in colour
Topics