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Methods in Molecular Biology

ELISA

Methods and Protocols

Editors: Hnasko, Robert (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2742-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.99
price for USA in USD
  • ISBN 978-1-4939-2741-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-5385-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.

Table of contents (19 chapters)

  • The Biochemical Properties of Antibodies and Their Fragments

    Hnasko, Robert M.

    Pages 1-14

  • Hybridoma Technology

    Hnasko, Robert M. (et al.)

    Pages 15-28

  • Affinity Purification of Antibodies

    Hnasko, Robert M. (et al.)

    Pages 29-41

  • Bioconjugation of Antibodies to Horseradish Peroxidase (HRP)

    Hnasko, Robert M.

    Pages 43-50

  • Indirect ELISA

    Lin, Alice V.

    Pages 51-59

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2742-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.99
price for USA in USD
  • ISBN 978-1-4939-2741-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-5385-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
ELISA
Book Subtitle
Methods and Protocols
Editors
  • Robert Hnasko
Series Title
Methods in Molecular Biology
Series Volume
1318
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2742-5
DOI
10.1007/978-1-4939-2742-5
Hardcover ISBN
978-1-4939-2741-8
Softcover ISBN
978-1-4939-5385-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 216
Number of Illustrations
9 b/w illustrations, 46 illustrations in colour
Topics