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Methods in Molecular Biology

Detection of Blotted Proteins

Methods and Protocols

Editors: Kurien, Biji T., Scofield, R. Hal (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-2718-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
Softcover $139.00
price for USA in USD
About this book

This detailed volume provides methods and techniques for detection after blotting.  Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.

Table of contents (38 chapters)

Table of contents (38 chapters)
  • Western Blotting: Origin and Ascent of the Species

    Pages 1-3

    Burnette, W. Neal

  • Methods to Concentrate Proteins for Protein Isolation, Proteomic, and Peptidomic Evaluation

    Pages 5-18

    Goldring, J. P. Dean

  • Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose

    Pages 19-25

    Goldring, J. P. Dean

  • Detection of Blotted Proteins: Not All Blockers Are Created Equal

    Pages 27-32

    Kothari, Vishal, M.Pharm. (et al.)

  • Protein Stains to Detect Antigen on Membranes

    Pages 33-40

    Dsouza, Anil, Ph.D. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-2718-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
Softcover $139.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Detection of Blotted Proteins
Book Subtitle
Methods and Protocols
Editors
  • Biji T. Kurien
  • R. Hal Scofield
Series Title
Methods in Molecular Biology
Series Volume
1314
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2718-0
DOI
10.1007/978-1-4939-2718-0
Hardcover ISBN
978-1-4939-2717-3
Softcover ISBN
978-1-4939-5595-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 386
Number of Illustrations
53 b/w illustrations, 48 illustrations in colour
Topics