Detection of Blotted Proteins
Methods and Protocols
Editors: Kurien, Biji T., Scofield, R. Hal (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Buy this book
- About this book
-
This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.
- Table of contents (38 chapters)
-
-
Western Blotting: Origin and Ascent of the Species
Pages 1-3
-
Methods to Concentrate Proteins for Protein Isolation, Proteomic, and Peptidomic Evaluation
Pages 5-18
-
Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose
Pages 19-25
-
Detection of Blotted Proteins: Not All Blockers Are Created Equal
Pages 27-32
-
Protein Stains to Detect Antigen on Membranes
Pages 33-40
-
Table of contents (38 chapters)
Recommended for you
Bibliographic Information
- Bibliographic Information
-
- Book Title
- Detection of Blotted Proteins
- Book Subtitle
- Methods and Protocols
- Editors
-
- Biji T. Kurien
- R. Hal Scofield
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1314
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2718-0
- DOI
- 10.1007/978-1-4939-2718-0
- Hardcover ISBN
- 978-1-4939-2717-3
- Softcover ISBN
- 978-1-4939-5595-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIV, 386
- Number of Illustrations
- 53 b/w illustrations, 48 illustrations in colour
- Topics
