Methods in Molecular Biology

Pyrosequencing

Methods and Protocols

Editors: Lehmann, Ulrich, Tost, Jorg (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-2715-9
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Softcover $139.99
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About this book

The primary purpose of this volume is to demonstrate the range of applications of the Pyrosequencing technology in research and diagnostics and to provide detailed protocols. Beginning with an up-to-date overview of the biochemistry, the volume continues with quantitative analysis of genetic variation, ratio of expressed alleles at the RNA level, analysis of DNA methylation, global DNA methylation assays, specialized applications for DNA methylation analysis including loss of imprinting, single blastocyst analysis, allele-specific DNA methylation patterns, DNA methylation patterns associated with specific histone modifications. The volume further details tools and protocols for the detection of viruses and bacteria, and genetic and epigenetic analyses for forensics using Pyrosequencing. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology.

Table of contents (27 chapters)

Table of contents (27 chapters)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-2715-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
Softcover $139.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Pyrosequencing
Book Subtitle
Methods and Protocols
Editors
  • Ulrich Lehmann
  • Jorg Tost
Series Title
Methods in Molecular Biology
Series Volume
1315
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2715-9
DOI
10.1007/978-1-4939-2715-9
Hardcover ISBN
978-1-4939-2714-2
Softcover ISBN
978-1-4939-4909-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 414
Number of Illustrations
26 b/w illustrations, 57 illustrations in colour
Topics