- Features techniques for researching multiple sclerosis on a molecular and cellular level
- Includes step-by-step methodologies that have been tested in labs around the world
- Provides key tips and implementation advice from the experts
Buy this book
- About this book
-
This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models. With so much effort needed to elucidate basic disease mechanisms, to clone disease relevant genes, to define novel biomarkers, and to discover novel and improved therapeutic and curative treatments, this book serves to aid researchers in accomplishing these enormous goals. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and easy to use, Multiple Sclerosis: Methods and Protocols will empower the reader to perform novel research regarding pathophysiology and treatment for MS.
- About the authors
-
Prof. Robert Weissert, M.D. Ph.D. University Professor of Clinical Neurobiology Department of Neurology University of Regensburg Universitätsstraße 84 93053 Regensburg Germany Phone: +49-941-9418721 E-mail: robert.weissert@klinik.uni-regensburg.de
- Table of contents (17 chapters)
-
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Positional Gene Cloning in Experimental Populations
Pages 3-24
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Generation of Transgenic Rats Using Lentiviral Vectors
Pages 25-37
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DNA Vaccination Techniques
Pages 39-50
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Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture
Pages 53-61
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ELISPOT Techniques
Pages 63-71
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Table of contents (17 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Multiple Sclerosis
- Book Subtitle
- Methods and Protocols
- Editors
-
- Robert Weissert
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1304
- Copyright
- 2016
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2630-5
- DOI
- 10.1007/978-1-4939-2630-5
- Hardcover ISBN
- 978-1-4939-2629-9
- Softcover ISBN
- 978-1-4939-4938-0
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 232
- Number of Illustrations
- 19 b/w illustrations, 18 illustrations in colour
- Topics
