- Assists academic and industrial researchers involved in drug and probe discovery to adopt label-free techniques
- Provides fundamental and practical methods from expert researchers
- Includes tips and key implementation advice to aid in translating techniques to your own lab
Buy this book
- About this book
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This volume explores label-free biosensors, advantageous in part because this technology bypasses the need of labels, reporters, and cell engineering, all of which are common to labeled techniques but may introduce artifacts in assay results. Addressing several fundamental and practical aspects as to how to implement label-free methods in the drug discovery process, this book covers a wide range of topics, including binding kinetics determination, fragment screening, antibody epitope mapping, protein-protein interaction profiling and screening, receptor pathway deconvolution, drug pharmacology profiling and screening, target identification, drug toxicity assessment, and physical phenotype profiling and diagnostics based on various cellular processes such as cell adhesion, migration, invasion, infection, and inflammation. As part of the Methods in Pharmacology and Toxicology series, chapters aim to provide key detail and implementation advice to aid with progress in the lab.
Practical and thorough, Label-Free Biosensor Methods in Drug Discovery provides a new avenue for rapid access to a focused collection of highly regarded contributions in the field.
- Table of contents (21 chapters)
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Label-Free Technologies: Which Technique to Use and What to Watch Out for!
Pages 3-15
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Label-Free Cell Phenotypic Profiling and Screening: Techniques, Experimental Design, and Data Assessment
Pages 17-33
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Surface Plasmon Resonance for Therapeutic Antibody Characterization
Pages 35-76
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Label-Free Cell-Based Biosensor Methods in Drug Toxicology Analysis
Pages 77-108
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Kinetics Characterization of Ligand–Receptor Interactions Using Oblique-Incidence Reflectivity Difference Method
Pages 111-118
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Table of contents (21 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Label-Free Biosensor Methods in Drug Discovery
- Editors
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- Ye Fang
- Series Title
- Methods in Pharmacology and Toxicology
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2617-6
- DOI
- 10.1007/978-1-4939-2617-6
- Hardcover ISBN
- 978-1-4939-2616-9
- Softcover ISBN
- 978-1-4939-4961-8
- Series ISSN
- 1557-2153
- Edition Number
- 1
- Number of Pages
- XI, 390
- Number of Illustrations
- 21 b/w illustrations, 93 illustrations in colour
- Topics
