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Methods in Molecular Biology

Rab GTPases

Methods and Protocols

Editors: Li, Guangpu (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-4939-2569-8
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-2568-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-5594-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling and endocytic Rabs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.

Instructive and practical, Rab GTPases: Methods and Protocols approaches each topic with great detail and is a valuable resource for researchers and students interested in the field of Rab GTPases.

About the authors

Guangpu Li, Ph.D. Department of Biochemistry and Molecular Biology University of Oklahoma Health Sciences Center 975 NE 10th Street, BRC 417B Oklahoma City, OK 73104 Tel: (405) 271-2227 ext. 61232 Fax: (405) 271-3910 E-mail: guangpu-li@ouhsc.edu

Table of contents (29 chapters)

Table of contents (29 chapters)
  • Rab Family of GTPases

    Pages 1-15

    Li, Guangpu (et al.)

  • Bioinformatic Approaches to Identifying and Classifying Rab Proteins

    Pages 17-28

    Diekmann, Yoan (et al.)

  • Rab-NANOPS: FRET Biosensors for Rab Membrane Nanoclustering and Prenylation Detection in Mammalian Cells

    Pages 29-45

    Najumudeen, Arafath Kaja (et al.)

  • High-Throughput Assay for Profiling the Substrate Specificity of Rab GTPase-Activating Proteins

    Pages 47-60

    Mishra, Ashwini K. (et al.)

  • Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts

    Pages 61-71

    Nottingham, Ryan M. (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2569-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-2568-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-5594-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Rab GTPases
Book Subtitle
Methods and Protocols
Editors
  • Guangpu Li
Series Title
Methods in Molecular Biology
Series Volume
1298
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2569-8
DOI
10.1007/978-1-4939-2569-8
Hardcover ISBN
978-1-4939-2568-1
Softcover ISBN
978-1-4939-5594-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 358
Number of Illustrations
28 b/w illustrations, 54 illustrations in colour
Topics