Methods in Molecular Biology

RNA Nanotechnology and Therapeutics

Methods and Protocols

Editors: Guo, Peixuan, Haque, Farzin (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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Hardcover $139.99
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Softcover $119.00
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About this book

This volume contains a compilation of techniques and laboratory protocols directly related to RNA nano technology and its applications in nano biotechnology and nano medicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nano architectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nano constructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.

About the authors

Peixuan Guo, Ph. D. William Farish Endowed Chair in Nano biotechnology Professor of School of Pharmacy Director of Nano biotechnology Center University of Kentucky Director of NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy Mailing address College of Pharmacy University of Kentucky 565 Biopharm Complex 789 S. Limestone Street Lexington, KY 40536 Phone: (Office) (859)218-0128 (Cell Phone): (513)728-1411 peixuan.guo@uky.edu (future)

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Overview of Methods in RNA Nanotechnology: Synthesis, Purification, and Characterization of RNA Nanoparticles

    Pages 1-19

    Haque, Farzin, Ph.D. (et al.)

  • Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-assembly

    Pages 21-42

    Lavelle, Christophe (et al.)

  • Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM

    Pages 43-57

    Koh, Kyung Duk (et al.)

  • Silver Nanoclusters for RNA Nanotechnology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies

    Pages 59-66

    Afonin, Kirill A. (et al.)

  • Large Scale Purification of RNA Nanoparticles by Preparative Ultracentrifugation

    Pages 67-82

    Jasinski, Daniel L. (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2562-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.99
price for USA in USD
  • ISBN 978-1-4939-2561-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-5450-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
RNA Nanotechnology and Therapeutics
Book Subtitle
Methods and Protocols
Editors
  • Peixuan Guo
  • Farzin Haque
Series Title
Methods in Molecular Biology
Series Volume
1297
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2562-9
DOI
10.1007/978-1-4939-2562-9
Hardcover ISBN
978-1-4939-2561-2
Softcover ISBN
978-1-4939-5450-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 239
Number of Illustrations
20 b/w illustrations, 49 illustrations in colour
Topics