- Includes cutting-edge methods and protocols on cAMP signaling
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from experts
Buy this book
- About this book
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This detailed volume encompasses new technological developments that specifically address questions related to adenosine 3′,5′-monophosphate (cAMP) compartmentalization, that probe relevant protein-protein interactions, that increase the spatial and temporal resolution of cAMP signal detection, and that can facilitate integration of the mounting complexity of the information that is becoming available on this signaling system. cAMP, the prototypical intracellular second messenger, regulates a large variety of cellular functions and biological processes, including gene transcription, cell metabolism, proliferation, development, as well as more specialized functions depending on the cell type, so the realization of its extremely complex spatial organization and local regulation is providing novel mechanistic insight into cell physiology and is producing a novel framework for the identification of disease mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, cAMP Signaling: Methods and Protocols serves as a vital resource for researchers working in this expanding, complex field.
- Table of contents (16 chapters)
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Simultaneous Assessment of cAMP Signaling Events in Different Cellular Compartments Using FRET-Based Reporters
Pages 1-12
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Recording Intracellular cAMP Levels with EPAC-Based FRET Sensors by Fluorescence Lifetime Imaging
Pages 13-24
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A Novel Approach Combining Real-Time Imaging and the Patch-Clamp Technique to Calibrate FRET-Based Reporters for cAMP in Their Cellular Microenvironment
Pages 25-40
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Structure-Based, In Silico Approaches for the Development of Novel cAMP FRET Reporters
Pages 41-58
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Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification
Pages 59-70
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Table of contents (16 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- cAMP Signaling
- Book Subtitle
- Methods and Protocols
- Editors
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- Manuela Zaccolo
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1294
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2537-7
- DOI
- 10.1007/978-1-4939-2537-7
- Hardcover ISBN
- 978-1-4939-2536-0
- Softcover ISBN
- 978-1-4939-4950-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- X, 222
- Number of Illustrations
- 11 b/w illustrations, 36 illustrations in colour
- Topics
