Methods in Molecular Biology

cAMP Signaling

Methods and Protocols

Editors: Zaccolo, Manuela (Ed.)

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  • Includes cutting-edge methods and protocols on cAMP signaling
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from experts
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  • ISBN 978-1-4939-2537-7
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Hardcover $139.99
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Softcover $119.00
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About this book

This detailed volume encompasses new technological developments that specifically address questions related to adenosine 3′,5′-monophosphate (cAMP) compartmentalization, that probe relevant protein-protein interactions, that increase the spatial and temporal resolution of cAMP signal detection, and that can facilitate integration of the mounting complexity of the information that is becoming available on this signaling system. cAMP, the prototypical intracellular second messenger, regulates a large variety of cellular functions and biological processes, including gene transcription, cell metabolism, proliferation, development, as well as more specialized functions depending on the cell type, so the realization of its extremely complex spatial organization and local regulation is providing novel mechanistic insight into cell physiology and is producing a novel framework for the identification of disease mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, cAMP Signaling: Methods and Protocols serves as a vital resource for researchers working in this expanding, complex field.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Simultaneous Assessment of cAMP Signaling Events in Different Cellular Compartments Using FRET-Based Reporters

    Pages 1-12

    Burdyga, Alex (et al.)

  • Recording Intracellular cAMP Levels with EPAC-Based FRET Sensors by Fluorescence Lifetime Imaging

    Pages 13-24

    Raspe, Marcel (et al.)

  • A Novel Approach Combining Real-Time Imaging and the Patch-Clamp Technique to Calibrate FRET-Based Reporters for cAMP in Their Cellular Microenvironment

    Pages 25-40

    Koschinski, Andreas (et al.)

  • Structure-Based, In Silico Approaches for the Development of Novel cAMP FRET Reporters

    Pages 41-58

    Machado, Matías (et al.)

  • Automated Image Analysis of FRET Signals for Subcellular cAMP Quantification

    Pages 59-70

    Leavesley, Silas J. (et al.)

Buy this book

eBook $89.00
price for USA in USD
  • ISBN 978-1-4939-2537-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover $139.99
price for USA in USD
Softcover $119.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
cAMP Signaling
Book Subtitle
Methods and Protocols
Editors
  • Manuela Zaccolo
Series Title
Methods in Molecular Biology
Series Volume
1294
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2537-7
DOI
10.1007/978-1-4939-2537-7
Hardcover ISBN
978-1-4939-2536-0
Softcover ISBN
978-1-4939-4950-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 222
Number of Illustrations
11 b/w illustrations, 36 illustrations in colour
Topics