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Methods in Molecular Biology

Mammary Stem Cells

Methods and Protocols

Editors: Vivanco, Maria del Mar (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-2519-3
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Hardcover $169.99
price for USA in USD
Softcover $139.99
price for USA in USD
About this book

The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.

About the authors

María dM Vivanco, PhD CIC bioGUNE Cell Biology & Stem Cells Unit Parque Tecnológico de Bizkaia, Ed 801A 48160 Derio (Bizkaia) Spain Tel: +34 94 406 1322 email: mdmvivanco@cicbiogune.es

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Breast Cancer Stem Cells: Current Advances and Clinical Implications

    Pages 1-49

    Luo, Ming (et al.)

  • A Protocol for Studying Embryonic Mammary Progenitor Cells During Mouse Mammary Primordial Development in Explant Culture

    Pages 51-62

    Kogata, Naoko (et al.)

  • FACS Sorting Mammary Stem Cells

    Pages 63-72

    Iriondo, Oihana (et al.)

  • Side Population

    Pages 73-81

    Behbod, Fariba, Pharm.D., Ph.D. (et al.)

  • Single-Cell Genome and Transcriptome Processing Prior to High-Throughput Sequencing

    Pages 83-114

    Aransay, Ana M. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-2519-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
Softcover $139.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Mammary Stem Cells
Book Subtitle
Methods and Protocols
Editors
  • Maria del Mar Vivanco
Series Title
Methods in Molecular Biology
Series Volume
1293
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2519-3
DOI
10.1007/978-1-4939-2519-3
Hardcover ISBN
978-1-4939-2518-6
Softcover ISBN
978-1-4939-4849-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 275
Number of Illustrations
13 b/w illustrations, 45 illustrations in colour
Topics