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Methods in Molecular Biology

Chromatin Protocols

Editors: Chellappan, Srikumar P. (Ed.)

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  • Includes new and revised cutting-edge methods and protocols for the study of chromatin
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2474-5
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  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-2473-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-4406-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin. Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Chromatin Protocols, Third Edition aims to help researchers in facilitating in-depth molecular analysis of various aspects of chromatin structure and function.

Table of contents (27 chapters)

Table of contents (27 chapters)
  • Beads-on-a-String on a Bead: Reconstitution and Analysis of Chromatin on a Solid Support

    Pages 1-14

    Sandaltzopoulos, Raphael (et al.)

  • Preparation and Analysis of Positioned Mononucleosomes

    Pages 15-26

    Kulaeva, Olga I. (et al.)

  • Chromatin Imaging with Time-Lapse Atomic Force Microscopy

    Pages 27-42

    Lyubchenko, Yuri L. (et al.)

  • Isolation of Specific Genomic Regions and Identification of Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using CRISPR

    Pages 43-52

    Fujita, Toshitsugu (et al.)

  • Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin

    Pages 53-66

    Gotoh, Eisuke, M.D., Ph.D.

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2474-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-2473-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-4406-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Chromatin Protocols
Editors
  • Srikumar P. Chellappan
Series Title
Methods in Molecular Biology
Series Volume
1288
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2474-5
DOI
10.1007/978-1-4939-2474-5
Hardcover ISBN
978-1-4939-2473-8
Softcover ISBN
978-1-4939-4406-4
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XIV, 492
Number of Illustrations
38 b/w illustrations, 36 illustrations in colour
Topics