- Includes cutting-edge methods and protocols
- Provides step-by-step details essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
The aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols, molecular modelling, and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.
- Table of contents (26 chapters)
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Affinity Chromatography: A Historical Perspective
Pages 1-19
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Protein Purification by Aminosquarylium Cyanine Dye-Affinity Chromatography
Pages 23-33
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One-Step Purification of Phosphinothricin Acetyltransferase Using Reactive Dye-Affinity Chromatography
Pages 35-42
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Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes
Pages 43-46
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Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography Using a Synthetic Aromatic Ligand
Pages 47-54
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Affinity Chromatography
- Book Subtitle
- Methods and Protocols
- Editors
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- Senta Reichelt
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1286
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2447-9
- DOI
- 10.1007/978-1-4939-2447-9
- Hardcover ISBN
- 978-1-4939-2446-2
- Softcover ISBN
- 978-1-4939-5584-8
- Series ISSN
- 1064-3745
- Edition Number
- 3
- Number of Pages
- XIII, 341
- Number of Illustrations
- 41 b/w illustrations, 37 illustrations in colour
- Topics
