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Methods in Molecular Biology

Coronaviruses

Methods and Protocols

Editors: Maier, Helena, Bickerton, Erica, Britton, Paul (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
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  • ISBN 978-1-4939-2438-7
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-2437-0
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  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
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  • ISBN 978-1-4939-4921-2
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About this book

This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • Coronaviruses: An Overview of Their Replication and Pathogenesis

    Pages 1-23

    Fehr, Anthony R. (et al.)

  • Identification of a Novel Coronavirus from Guinea Fowl Using Metagenomics

    Pages 27-31

    Ducatez, Mariette F. (et al.)

  • Serological Diagnosis of Feline Coronavirus Infection by Immunochromatographic Test

    Pages 33-39

    Takano, Tomomi (et al.)

  • Estimation of Evolutionary Dynamics and Selection Pressure in Coronaviruses

    Pages 41-48

    Munir, Muhammad (et al.)

  • The Preparation of Chicken Tracheal Organ Cultures for Virus Isolation, Propagation, and Titration

    Pages 51-56

    Hennion, Ruth M.

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2438-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-2437-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-4921-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Coronaviruses
Book Subtitle
Methods and Protocols
Editors
  • Helena Maier
  • Erica Bickerton
  • Paul Britton
Series Title
Methods in Molecular Biology
Series Volume
1282
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2438-7
DOI
10.1007/978-1-4939-2438-7
Hardcover ISBN
978-1-4939-2437-0
Softcover ISBN
978-1-4939-4921-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 285
Number of Illustrations
15 b/w illustrations, 27 illustrations in colour
Topics