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Protein-Protein Interactions

Methods and Applications

  • Book
  • © 2015

Overview

Editors:
  1. Cheryl L. Meyerkord

    1. Department of Pharmacology and Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, USA

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  2. Haian Fu

    1. Department of Pharmacology, Department of Hematology & Medical Oncology, and Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1278)

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Table of contents (39 protocols)

  1. Front Matter

    Pages i-xv
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  2. Overviews

    1. Front Matter

      Pages 1-1
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    2. Structural Basis of Protein-Protein Interactions

      • Robert C. Liddington
      Pages 3-22

    3. Quantitative Analysis of Protein-Protein Interactions

      • Ziad M. Eletr, Keith D. Wilkinson
      Pages 23-37

    4. Protein-Protein Interaction Databases

      • Damian Szklarczyk, Lars Juhl Jensen
      Pages 39-56

    5. Computational Prediction of Protein-Protein Interactions

      • Tobias Ehrenberger, Lewis C. Cantley, Michael B. Yaffe
      Pages 57-75

    6. Structure-Based Computational Approaches for Small-Molecule Modulation of Protein-Protein Interactions

      • David Xu, Bo Wang, Samy O. Meroueh
      Pages 77-92

    7. Targeting Protein-Protein Interactions for Drug Discovery

      • David C. Fry
      Pages 93-106

  3. Label-Free Biosensors and Techniques

    1. Front Matter

      Pages 107-107
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    2. Studying Protein-Protein Interactions Using Surface Plasmon Resonance

      • Zaneta Nikolovska-Coleska
      Pages 109-138

    3. Resonant Waveguide Grating for Monitoring Biomolecular Interactions

      • Meng Wu, Min Li
      Pages 139-152

    4. Quartz Microbalance Technology for Probing Biomolecular Interactions

      • Gabriella T. Heller, Alison R. Mercer-Smith, Malkiat S. Johal
      Pages 153-164

    5. Label-Free Kinetic Analysis of an Antibody–Antigen Interaction Using Biolayer Interferometry

      • Sriram Kumaraswamy, Renee Tobias
      Pages 165-182

    6. Characterization of Protein-Protein Interactions by Isothermal Titration Calorimetry

      • Adrian Velazquez-Campoy, Stephanie A. Leavitt, Ernesto Freire
      Pages 183-204

    7. Sedimentation Equilibrium Studies

      • Ian A. Taylor, Katrin Rittinger, John F. Eccleston
      Pages 205-222

    8. Detecting Protein-Protein Interactions by Gel Filtration Chromatography

      • Yan Bai
      Pages 223-232

    9. Using Light Scattering to Determine the Stoichiometry of Protein Complexes

      • Jeremy Mogridge
      Pages 233-238

    10. Circular Dichroism (CD) Analyses of Protein-Protein Interactions

      • Norma J. Greenfield
      Pages 239-265

    11. Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy

      • Peter M. Thompson, Moriah R. Beck, Sharon L. Campbell
      Pages 267-279

    12. Quantitative Protein Analysis by Mass Spectrometry

      • Vishwajeeth R. Pagala, Anthony A. High, Xusheng Wang, Haiyan Tan, Kiran Kodali, Ashutosh Mishra et al.
      Pages 281-305
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Keywords

About this book

The second edition covers a wide range of protein-protein interaction detection topics. Protein-Protein Interactions: Methods and Applications focuses on core technological platforms used to study protein-protein interactions and cutting-edge technologies that reflect recent scientific advances and the emerging focus on therapeutic discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. These well-detailed protocols describe methods for identifying protein-protein interaction partners, analyzing of protein-protein interactions quantitatively and qualitatively, monitoring protein-protein interactions in live cells, and predicting and determining interaction interfaces.

Authoritative and cutting-edge, Protein-Protein Interactions: Methods and Applications, Second Edition is a valuable resource that will enable readers to elucidate the mechanisms of protein-protein interactions, determine the role of these interactions in diverse biological processes, and target protein-protein interactions for therapeutic discovery.

Editors and Affiliations

  • Department of Pharmacology and Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, USA

    Cheryl L. Meyerkord

  • Department of Pharmacology, Department of Hematology & Medical Oncology, and Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, USA

    Haian Fu

Bibliographic Information

  • Book Title: Protein-Protein Interactions

  • Book Subtitle: Methods and Applications

  • Editors: Cheryl L. Meyerkord, Haian Fu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2425-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2424-0Published: 10 April 2015

  • Softcover ISBN: 978-1-4939-4985-4Published: 29 October 2016

  • eBook ISBN: 978-1-4939-2425-7Published: 09 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 620

  • Number of Illustrations: 69 b/w illustrations, 70 illustrations in colour

  • Topics: Protein Science

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