Bacterial Transcriptional Control
Methods and Protocols
Editors: Artsimovitch, Irina, Santangelo, Thomas J. (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls.
Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future.
- About the authors
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Name: Irina Artsimovitch Address: Department of Microbiology The Ohio State University 484 West 12th Avenue Columbus, OH 43210 Phone: (614) 292-6777 Email: artsimovitch.1@osu.edu
- Table of contents (20 chapters)
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Mapping the Escherichia coli Transcription Elongation Complex with Exonuclease III
Pages 1-12
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Purification of Bacterial RNA Polymerase: Tools and Protocols
Pages 13-29
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Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues
Pages 31-51
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In Vitro and In Vivo Methodologies for Studying the Sigma 54-Dependent Transcription
Pages 53-79
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Methods for the Assembly and Analysis of In Vitro Transcription-Coupled-to-Translation Systems
Pages 81-99
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Table of contents (20 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Bacterial Transcriptional Control
- Book Subtitle
- Methods and Protocols
- Editors
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- Irina Artsimovitch
- Thomas J. Santangelo
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1276
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2392-2
- DOI
- 10.1007/978-1-4939-2392-2
- Hardcover ISBN
- 978-1-4939-2391-5
- Softcover ISBN
- 978-1-4939-5467-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 342
- Number of Illustrations
- 23 b/w illustrations, 25 illustrations in colour
- Topics
