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Methods in Molecular Biology

Bacterial Transcriptional Control

Methods and Protocols

Editors: Artsimovitch, Irina, Santangelo, Thomas J. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2392-2
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Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-2391-5
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  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
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  • ISBN 978-1-4939-5467-4
  • Free shipping for individuals worldwide
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About this book

This volume is designed to be a resource of proven techniques and approaches for probing the activities of bacterial, eukaryotic, and archaeal RNA polymerases. This book features a collection of in vitro and in vivo technologies that will permit researchers to purify and probe the position and stability of RNA polymerase complexes at different points of the transcription cycle, analyze the various translocations and intermolecular movements associated with catalysis, define recruitment strategies, probe the roles of transcription factors in each stage of the cycle, highlight conserved and disparate fidelity mechanisms, analyze the resultant transcripts, and study coordination of the nascent mRNA synthesis by the RNA polymerase and mRNA translation by the ribosome. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls.

Practical and timely, Bacterial Transcriptional Controls: Methods and Protocols highlights the breadth and depth of techniques that are likely to continue shaping the transcription community in the future.

About the authors

Name: Irina Artsimovitch Address: Department of Microbiology The Ohio State University 484 West 12th Avenue Columbus, OH 43210 Phone: (614) 292-6777 Email: artsimovitch.1@osu.edu

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Mapping the Escherichia coli Transcription Elongation Complex with Exonuclease III

    Pages 1-12

    Liu, Zhaokun (et al.)

  • Purification of Bacterial RNA Polymerase: Tools and Protocols

    Pages 13-29

    Svetlov, Vladimir (et al.)

  • Monitoring Translocation of Multisubunit RNA Polymerase Along the DNA with Fluorescent Base Analogues

    Pages 31-51

    Malinen, Anssi M. (et al.)

  • In Vitro and In Vivo Methodologies for Studying the Sigma 54-Dependent Transcription

    Pages 53-79

    Buck, Martin (et al.)

  • Methods for the Assembly and Analysis of In Vitro Transcription-Coupled-to-Translation Systems

    Pages 81-99

    Castro-Roa, Daniel (et al.)

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2392-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-2391-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-5467-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bacterial Transcriptional Control
Book Subtitle
Methods and Protocols
Editors
  • Irina Artsimovitch
  • Thomas J. Santangelo
Series Title
Methods in Molecular Biology
Series Volume
1276
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2392-2
DOI
10.1007/978-1-4939-2392-2
Hardcover ISBN
978-1-4939-2391-5
Softcover ISBN
978-1-4939-5467-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 342
Number of Illustrations
23 b/w illustrations, 25 illustrations in colour
Topics