- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
-
This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.
- Table of contents (15 chapters)
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Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes
Pages 1-16
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Primer Design for PCR Reactions in Forensic Biology
Pages 17-30
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Design of Primers and Probes for Quantitative Real-Time PCR Methods
Pages 31-56
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Large-Scale Nucleotide Sequence Alignment and Sequence Variability Assessment to Identify the Evolutionarily Highly Conserved Regions for Universal Screening PCR Assay Design: An Example of Influenza A Virus
Pages 57-72
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Low-Concentration Initiator Primers Improve the Amplification of Gene Targets with High Sequence Variability
Pages 73-89
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Table of contents (15 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- PCR Primer Design
- Editors
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- Chhandak Basu
- Series Title
- Methods in Molecular Biology
- Series Volume
- 1275
- Copyright
- 2015
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media New York
- eBook ISBN
- 978-1-4939-2365-6
- DOI
- 10.1007/978-1-4939-2365-6
- Hardcover ISBN
- 978-1-4939-2364-9
- Softcover ISBN
- 978-1-4939-4755-3
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XII, 216
- Number of Illustrations
- 28 b/w illustrations, 45 illustrations in colour
- Topics
