Methods in Molecular Biology

PCR Primer Design

Editors: Basu, Chhandak (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-4939-2365-6
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Hardcover $179.99
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Softcover $139.99
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About this book

This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes

    Pages 1-16

    Andreson, Reidar (et al.)

  • Primer Design for PCR Reactions in Forensic Biology

    Pages 17-30

    Elkins, Kelly M., Ph.D.

  • Design of Primers and Probes for Quantitative Real-Time PCR Methods

    Pages 31-56

    Rodríguez, Alicia (et al.)

  • Large-Scale Nucleotide Sequence Alignment and Sequence Variability Assessment to Identify the Evolutionarily Highly Conserved Regions for Universal Screening PCR Assay Design: An Example of Influenza A Virus

    Pages 57-72

    Nagy, Alexander (et al.)

  • Low-Concentration Initiator Primers Improve the Amplification of Gene Targets with High Sequence Variability

    Pages 73-89

    Pierce, Kenneth E. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-2365-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase Institutional customers should get in touch with their account manager
Hardcover $179.99
price for USA in USD
Softcover $139.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
PCR Primer Design
Editors
  • Chhandak Basu
Series Title
Methods in Molecular Biology
Series Volume
1275
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2365-6
DOI
10.1007/978-1-4939-2365-6
Hardcover ISBN
978-1-4939-2364-9
Softcover ISBN
978-1-4939-4755-3
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 216
Number of Illustrations
28 b/w illustrations, 45 illustrations in colour
Topics