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Methods in Molecular Biology

Rhodopsin

Methods and Protocols

Editors: Jastrzebska, Beata (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume explores the technological improvements in protein engineering, expression, purification, and crystallization of several rhodopsin photoactive intermediates, thus increasing our understanding of rhodopsin activation. The first chapters of the book focus on methods developed to study fundamentals of rhodopsin structure and function, starting with improved purification protocols of native and mutated rhodopsin, followed by methods used for rhodopsin reconstitution into lipid bilayers stabilizing rhodopsin function properties, and finally describing recently developed methods to study structural dynamics of rhodopsin activation and its mechanistic properties. Subsequently, chapters underline various techniques that have been developed to visualize the rhodopsin dimer and to study its functional significance.  The next few chapters highlight cutting-edge imaging techniques of photoreceptors, rhodopsin trafficking, and its diffusion within signaling membranes. Finally the book concludes with recent developments that could be potentially beneficial in patient treatments, and treatment strategies for retinal degenerative diseases. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Timely and practical, Rhodopsin: Methods and Protocols reaches out to researchers and health practitioners, and provides timely protocol useful for studying  structural and functional properties of rhodopsin.

About the authors

Beata Jastrzebska, Ph.D. Department of Pharmacology, W317 Case Western Reserve University 10900 Euclid Avenue Cleveland, OH 44106-4965

Table of contents (25 chapters)

Table of contents (25 chapters)
  • The G Protein-Coupled Receptor Rhodopsin: A Historical Perspective

    Pages 3-18

    Hofmann, Lukas (et al.)

  • Rhodopsin Purification from Dark-Adapted Bovine Retina

    Pages 21-38

    Blankenship, Elise (et al.)

  • Mammalian Expression, Purification, and Crystallization of Rhodopsin Variants

    Pages 39-54

    Mattle, Daniel (et al.)

  • Imaging of Rhodopsin Crystals with Two-Photon Microscopy

    Pages 55-64

    Palczewska, Grazyna, M.Sc., M.E. (et al.)

  • Functional Stability of Rhodopsin in a Bicelle System: Evaluating G Protein Activation by Rhodopsin in Bicelles

    Pages 67-76

    Kaya, Ali I. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-2330-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-2329-8
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA in USD
  • ISBN 978-1-4939-4936-6
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Rhodopsin
Book Subtitle
Methods and Protocols
Editors
  • Beata Jastrzebska
Series Title
Methods in Molecular Biology
Series Volume
1271
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2330-4
DOI
10.1007/978-1-4939-2330-4
Hardcover ISBN
978-1-4939-2329-8
Softcover ISBN
978-1-4939-4936-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 414
Number of Illustrations
23 b/w illustrations, 71 illustrations in colour
Topics

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