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Methods in Molecular Biology

Nuclear Bodies and Noncoding RNAs

Methods and Protocols

Editors: Nakagawa, Shinichi, Hirose, Tetsuro (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2253-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA in USD
  • ISBN 978-1-4939-2252-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-5549-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume focuses on cytological, biochemical, and molecular biological methods to identify and examine the function of each nuclear body, with an emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that reveal how certain long non protein-coding RNAs accumulate in specific nuclear bodies and regulate the function of the bodies by serving as architectural components or controlling the dynamics of associating protein components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nuclear Bodies and Noncoding RNAs: Methods and Protocols serves as a guideline for further study into the sophisticated regulation of gene expression.

Table of contents (21 chapters)

  • Visualization of lncRNA by Single-Molecule Fluorescence In Situ Hybridization

    Dunagin, Margaret (et al.)

    Pages 3-19

  • Super-Resolution Imaging of Nuclear Bodies by STED Microscopy

    Okada, Yasushi (et al.)

    Pages 21-35

  • High-Resolution 3D DNA FISH Using Plasmid Probes and Computational Correction of Optical Aberrations to Study Chromatin Structure at the Sub-megabase Scale

    Giorgetti, Luca (et al.)

    Pages 37-53

  • Time-Lapse Imaging of Nuclear Bodies

    Hutten, Saskia (et al.)

    Pages 55-67

  • Visualization of Nucleic Acids with Synthetic Exciton-Controlled Fluorescent Oligonucleotide Probes

    Wang, Dan Ohtan (et al.)

    Pages 69-87

Buy this book

eBook $89.00
price for USA in USD (gross)
  • ISBN 978-1-4939-2253-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $119.00
price for USA in USD
  • ISBN 978-1-4939-2252-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $119.00
price for USA in USD
  • ISBN 978-1-4939-5549-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Nuclear Bodies and Noncoding RNAs
Book Subtitle
Methods and Protocols
Editors
  • Shinichi Nakagawa
  • Tetsuro Hirose
Series Title
Methods in Molecular Biology
Series Volume
1262
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2253-6
DOI
10.1007/978-1-4939-2253-6
Hardcover ISBN
978-1-4939-2252-9
Softcover ISBN
978-1-4939-5549-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 351
Number of Illustrations
24 b/w illustrations, 46 illustrations in colour
Topics