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Structural Proteomics

High-Throughput Methods

  • Book
  • © 2015

Overview

Editors:
  1. Raymond J. Owens

    1. Oxford Protein Production Facility UK, Didcot, United Kingdom

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols on structural proteomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1261)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Bioinformatics and Data Management

    1. Front Matter

      Pages 1-1
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    2. Protein Structure Annotation Resources

      • Margaret J. Gabanyi, Helen M. Berman
      Pages 3-20

    3. PiMS: A Data Management System for Structural Proteomics

      • Chris Morris
      Pages 21-34

    4. Prediction and Analysis of Intrinsically Disordered Proteins

      • Marco Punta, István Simon, Zsuzsanna Dosztányi
      Pages 35-59

  3. Soluble Proteins

    1. Front Matter

      Pages 61-61
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    2. Characterization and Production of Protein Complexes by Co-expression in Escherichia coli

      • Matthias Haffke, Martin Marek, Martin Pelosse, Marie-Laure Diebold, Uwe Schlattner, Imre Berger et al.
      Pages 63-89

    3. The Production of Multiprotein Complexes in Insect Cells Using the Baculovirus Expression System

      • Wassim Abdulrahman, Laura Radu, Frederic Garzoni, Olga Kolesnikova, Kapil Gupta, Judit Osz-Papai et al.
      Pages 91-114

    4. Production of Cell Surface and Secreted Glycoproteins in Mammalian Cells

      • Elena Seiradake, Yuguang Zhao, Weixian Lu, A. Radu Aricescu, E. Yvonne Jones
      Pages 115-127

    5. Cell-Free Protein Synthesis Systems Derived from Cultured Mammalian Cells

      • Andreas K. Brödel, Doreen A. Wüstenhagen, Stefan Kubick
      Pages 129-140

    6. Crystallization: Digging into the Past to Learn Lessons for the Future

      • Vincent J. Fazio, Thomas S. Peat, Janet Newman
      Pages 141-156

  4. Membrane Proteins

    1. Front Matter

      Pages 157-157
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    2. Screening of Stable G-Protein-Coupled Receptor Variants in Saccharomyces cerevisiae

      • Mitsunori Shiroishi, Takuya Kobayashi
      Pages 159-170

    3. Cell-Free Expression of G-Protein-Coupled Receptors

      • Erika Orbán, Davide Proverbio, Stefan Haberstock, Volker Dötsch, Frank Bernhard
      Pages 171-195

    4. GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System

      • Nien-Jen Hu, Heather Rada, Nahid Rahman, Joanne E. Nettleship, Louise Bird, So Iwata et al.
      Pages 197-209

    5. Methods for the Successful Crystallization of Membrane Proteins

      • Isabel Moraes, Margarida Archer
      Pages 211-230

  5. Structural Characterization of Proteins

    1. Front Matter

      Pages 231-231
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    2. Application of In Situ Diffraction in High-Throughput Structure Determination Platforms

      • Pierre Aller, Juan Sanchez-Weatherby, James Foadi, Graeme Winter, Carina M. C. Lobley, Danny Axford et al.
      Pages 233-253

    3. CD Spectroscopy: An Essential Tool for Quality Control of Protein Folding

      • Giuliano Siligardi, Rohanah Hussain
      Pages 255-276

    4. High-Throughput Studies of Protein Shapes and Interactions by Synchrotron Small-Angle X-Ray Scattering

      • Cy M. Jeffries, Dmitri I. Svergun
      Pages 277-301
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Keywords

About this book

This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins, notably integral membrane proteins and multi-protein complexes. Structural Proteomics: High-Throughput Methods, Second Edition begins by exploring the resources available for curation, annotation, and structure prediction in silico, and continues with methods for sample preparation of both proteins and crystals, as well as structural characterization techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Structural Proteomics: High-Throughput Methods, Second Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.

Editors and Affiliations

  • Oxford Protein Production Facility UK, Didcot, United Kingdom

    Raymond J. Owens

Bibliographic Information

  • Book Title: Structural Proteomics

  • Book Subtitle: High-Throughput Methods

  • Editors: Raymond J. Owens

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2230-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2229-1Published: 11 December 2014

  • Softcover ISBN: 978-1-4939-4832-1Published: 10 September 2016

  • eBook ISBN: 978-1-4939-2230-7Published: 10 December 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 375

  • Number of Illustrations: 27 b/w illustrations, 65 illustrations in colour

  • Topics: Protein Science, Protein Structure

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