Methods in Molecular Biology

Insoluble Proteins

Methods and Protocols

Editors: García-Fruitós, Elena (Ed.)

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  • Includes cutting-edge methods and protocols involving insoluble proteins
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-2205-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
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  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
Softcover $139.00
price for USA in USD
About this book

With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • General Introduction: Recombinant Protein Production and Purification of Insoluble Proteins

    Pages 1-24

    Ferrer-Miralles, Neus (et al.)

  • Overcoming the Solubility Problem in E. coli: Available Approaches for Recombinant Protein Production

    Pages 27-44

    Correa, Agustín (et al.)

  • Optimization of Culture Parameters and Novel Strategies to Improve Protein Solubility

    Pages 45-63

    Arya, Ranjana (et al.)

  • Cleavable Self-Aggregating Tags (cSAT) for Protein Expression and Purification

    Pages 65-78

    Lin, Zhanglin (et al.)

  • Beyond the Cytoplasm of Escherichia coli: Localizing Recombinant Proteins Where You Want Them

    Pages 79-97

    Boock, Jason T. (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-2205-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
Softcover $139.00
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Insoluble Proteins
Book Subtitle
Methods and Protocols
Editors
  • Elena García-Fruitós
Series Title
Methods in Molecular Biology
Series Volume
1258
Copyright
2015
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4939-2205-5
DOI
10.1007/978-1-4939-2205-5
Hardcover ISBN
978-1-4939-2204-8
Softcover ISBN
978-1-4939-4675-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 425
Number of Illustrations
31 b/w illustrations, 41 illustrations in colour
Topics